Transcriptoma das células da granulosa durante o desenvolvimento folicular em bovinos
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Data
2021-12-20
Autores
Mogollón García, Henry David
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
Os mecanismos pelos quais ocorrem o desenvolvimento folicular e o estabelecimento da dominância ainda são apenas parcialmente compreendidos, constituindo‐se como peças‐chave para o completo entendimento não apenas da fisiologia reprodutiva, como também das patologias. O objetivo do estudo foi descrever, holisticamente, a expressão diferencial de genes nos estágios foliculares de pré‐desvio, desvio, pósdesvio e pré‐ovulatorio. Um total de 1805 genes foram diferencialmente expressos (DEGs). Desses DEGs, cento e cinco apresentaram intersecção nos diferentes estágios foliculares. A análise em conjunto da ontologia gênica (GO), enriquecimento em conjunto de genes (GSEA) e a interação de proteína‐proteína (IPP), mostrou que os DEGs se associaram a respostas esteroidogênicas, proliferação celular e processos vasculares no folículo durante o estágio de desvio. No folículo pós‐desvio e pré‐ovulatorio, esses processos mantiveram estreita relação com a resposta inflamatória; fenômeno que foi liderado, principalmente, por DEGs da família quinase. Moléculas como Fig‐α, kit‐ligante, FGFs e seus receptores, GDF‐9 e BMP‐15, IGFs, ativina, EGF, KGF, peptídeo intestinal vasoativo e angiotensina‐II foram identificadas em vários estudos prévios e são relacionadas a numerosas atividades no contexto da dinâmica ovariana como a esteroidogênese, o crescimento e apoptose celular e a angiogênese. O nosso estudo complementou esses achados e mostrou a importância de DEGs que ainda não tinham sido descritos. Dentre esses DEGs, destacam‐se a ADM, NPR1, NPR3, CSF1R, RAGE. Em conclusão, os nossos resultados evidenciaram que a expressão do gene ADM, NPR1 e 3 é diferente entre o folículo desvio um e dois, regulando a vascularização e processos de desenvolvimento. Adicionalmente, a resposta inflamatória entre o folículo pós‐desvio e o folículo pré‐ovulatorio foi caracterizada pela expressão de genes agrupados no termo ontológico de RAGE e família das quinases, respectivamente.
The mechanisms by which follicular development and the establishment of dominance occur are still partially understood, constituting key elements for a complete understanding not only of reproductive physiology but also of pathologies. The aim of the study was to describe, holistically, the differential expression of genes in the pre‐deviation, deviation, post‐deviation, and pre‐ovulatory follicular stages. A total of 1805 genes were differentially expressed (DEGs). One hundred and five DEGs presented intersections in the different follicular stages. Together, gene ontology (GO), pooled gene enrichment (GSEA) and protein‐protein interaction (IPP) showed that DEGs were associated with steroidogenic responses, cell proliferation, and vascular processes in the follicle during the deviation. In the post‐deviation and pre‐ovulatory follicles, these processes were closely related to the inflammatory response; a phenomenon that was led mainly by DEGs of the kinase family. Molecules such as Fig‐ α, kit ligand, FGFs, and their receptors, GDF‐9 and BMP‐15, IGFs, activin, EGF, KGF, vasoactive intestinal peptide, and angiotensin‐II were identified in several previous studies and are related to numerous activities in the context of ovarian dynamics, notably steroidogenesis, follicle growth and apoptosis, and angiogenesis. Our study complemented these findings and showed the importance of DEGs that had not yet been described. Among these DEGs, were relevant ADM, NPR1, NPR3 and CSF1R. In conclusion, our results showed that the expression of the gene ADM, NPR1, and 3 is different between follicle deviation one and two, regulating vascularization and developmental processes. Additionally, the inflammatory response between the postdeviation follicle and the pre‐ovulatory follicle was characterized by the expression of genes grouped in the ontological term of RAGE and kinase family, respectively.
The mechanisms by which follicular development and the establishment of dominance occur are still partially understood, constituting key elements for a complete understanding not only of reproductive physiology but also of pathologies. The aim of the study was to describe, holistically, the differential expression of genes in the pre‐deviation, deviation, post‐deviation, and pre‐ovulatory follicular stages. A total of 1805 genes were differentially expressed (DEGs). One hundred and five DEGs presented intersections in the different follicular stages. Together, gene ontology (GO), pooled gene enrichment (GSEA) and protein‐protein interaction (IPP) showed that DEGs were associated with steroidogenic responses, cell proliferation, and vascular processes in the follicle during the deviation. In the post‐deviation and pre‐ovulatory follicles, these processes were closely related to the inflammatory response; a phenomenon that was led mainly by DEGs of the kinase family. Molecules such as Fig‐ α, kit ligand, FGFs, and their receptors, GDF‐9 and BMP‐15, IGFs, activin, EGF, KGF, vasoactive intestinal peptide, and angiotensin‐II were identified in several previous studies and are related to numerous activities in the context of ovarian dynamics, notably steroidogenesis, follicle growth and apoptosis, and angiogenesis. Our study complemented these findings and showed the importance of DEGs that had not yet been described. Among these DEGs, were relevant ADM, NPR1, NPR3 and CSF1R. In conclusion, our results showed that the expression of the gene ADM, NPR1, and 3 is different between follicle deviation one and two, regulating vascularization and developmental processes. Additionally, the inflammatory response between the postdeviation follicle and the pre‐ovulatory follicle was characterized by the expression of genes grouped in the ontological term of RAGE and kinase family, respectively.
Descrição
Palavras-chave
Vacas, Foliculogênese, Expressão gênica, Ovário, Cows, Folliculogenesis, Gene expression, Ovary