Influência do feijão na remodelação cardíaca após o infarto agudo do miocárdio em ratos
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Data
2021-12-16
Autores
Vieira, Nayane Maria
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Universidade Estadual Paulista (Unesp)
Resumo
Introdução: a insuficiência cardíaca é uma frequente complicação após o infarto agudo do miocárdio (IAM), sendo que a remodelação cardíaca (RC) tem papel de destaque na disfunção ventricular pós-infarto. Mecanismos como estresse oxidativo, inflamação e fibrose modulam esse processo, visando proteção cardiovascular, afim de reduzir tamanho do infarto e preservar a função cardíaca, pode-se destacar alimentos com propriedades antioxidantes, como o feijão (Phaseolus vulgaris). Objetivo: avaliar a influência da suplementação da farinha de feijão na remodelação cardíaca após o IAM em ratos. Material e Métodos: ratos Wistar machos, com peso entre 200 e 250g, foram submetidos ao infarto experimental ou à cirurgia simulada e alocados em 3 grupos: 1) Sham alimentado com dieta padrão (SC); 2) IAM alimentado com dieta padrão (IC) e 3) IAM alimentado com dieta suplementada com farinha de feijão (IF) 15%. Após 3 meses os animais foram submetidos ao estudo funcional, morfométrico e bioquímico. Foi realizado o teste de análise de variância de 1 via (ANOVA), o nível de significância adotado foi de 5%. Resultados: houve perda de 48 animais dos infartados, sendo que, 3 animais foram descartados, pois não foi induzido o modelo de infarto, e 1 animal no ecocardiograma inicial. Durante os 3 meses de experimento houve perda de 2 animais do grupo IC, e 2 animais do grupo IF. O tamanho médio dos infartos foi de 38% para o grupo IC, e 40% para o grupo IF. Não houve diferença entre a ingestão de ração e ganho de peso dos animais, o peso dos órgãos, do ventrículo esquerdo (VE) e ventrículo direito (VD) foram maiores nos animais com IAM. O IAM induziu alterações no VE, observados pelas variáveis estruturais e funcionais do ecocardiograma. Na análise de coração isolado, as variáveis de derivada positiva pressão-tempo (+dP/dtmax) e a derivada negativa de pressão-tempo (–dP/dtmax) foram menores entre os grupos IAM, enquanto a área sobre a curva (ASC) foi elevada nos animais com IAM. A área da secção transversa de miócitos (AST) foi maior nos grupos IAM, no estresse oxidativo, não foi possível observar diferença na carbonilação proteica total, além disso, não houve diferença nas expressões das proteínas do NRF-2 e colágeno III. Conclusão: a suplementação de farinha de feijão não atenuou a RC induzida pelo IAM em ratos.
Introduction: cardiac failure is a frequent complication after acute myocardial infarction (AMI), and cardiac remodeling (CR) plays an important role in postinfarction ventricular dysfunction. Mechanisms such as oxidative stress, inflammation and fibrosis modulate this process, aiming at cardiovascular protection, in order to reduce infarction size and preserve cardiac function, one can highlight foods with antioxidant properties, such as beans (Phaseolus vulgaris). Objective: to evaluate the influence of bean flour supplementation on cardiac remodeling after AMI in rats. Material and Methods: male Wistar rats, weighing between 200 and 250g, underwent experimental infarction or sham surgery and were allocated into 3 groups: 1) Sham fed a standard diet (SC); 2) AMI fed a standard diet (CI) and 3) AMI fed a diet supplemented with bean flour (IF) 15%. After 3 months the animals were submitted to a functional, morphometric and biochemical study. The 1-way analysis of variance test (ANOVA) was performed, the significance level adopted was 5%. Results: there was a loss of 48 animals from the infarctions, and 3 animals were discarded because the infarction model was not induced, and 1 animal in the initial echocardiogram. During the 3 months of the experiment there was a loss of 2 animals from the IC group, and 2 animals from the IF group. The mean infarct size was 38% for the IC group and 40% for the IF group. There was no difference between feed intake and weight gain of the animals, the weight of organs, left ventricle (LV) and right ventricle (RV) were higher in animals with AMI. The AMI induced changes in the LV, observed by the structural and functional variables of the echocardiogram. In the isolated heart analysis, the variables of positive pressure-time derivative (+dP/dtmax) and negative pressure-time derivative (– dP/dtmax) were lower between the AMI groups, while the area under the curve (AUC) was elevated in animals with AMI. The myocyte cross-sectional area (AST) was higher in the AMI groups, under oxidative stress, it was not possible to observe a difference in total protein carbonylation, in addition, there was no difference in the expressions of NRF-2 and collagen III proteins. Conclusion: bean flour supplementation did not attenuate the CR induced by AMI in rats.
Introduction: cardiac failure is a frequent complication after acute myocardial infarction (AMI), and cardiac remodeling (CR) plays an important role in postinfarction ventricular dysfunction. Mechanisms such as oxidative stress, inflammation and fibrosis modulate this process, aiming at cardiovascular protection, in order to reduce infarction size and preserve cardiac function, one can highlight foods with antioxidant properties, such as beans (Phaseolus vulgaris). Objective: to evaluate the influence of bean flour supplementation on cardiac remodeling after AMI in rats. Material and Methods: male Wistar rats, weighing between 200 and 250g, underwent experimental infarction or sham surgery and were allocated into 3 groups: 1) Sham fed a standard diet (SC); 2) AMI fed a standard diet (CI) and 3) AMI fed a diet supplemented with bean flour (IF) 15%. After 3 months the animals were submitted to a functional, morphometric and biochemical study. The 1-way analysis of variance test (ANOVA) was performed, the significance level adopted was 5%. Results: there was a loss of 48 animals from the infarctions, and 3 animals were discarded because the infarction model was not induced, and 1 animal in the initial echocardiogram. During the 3 months of the experiment there was a loss of 2 animals from the IC group, and 2 animals from the IF group. The mean infarct size was 38% for the IC group and 40% for the IF group. There was no difference between feed intake and weight gain of the animals, the weight of organs, left ventricle (LV) and right ventricle (RV) were higher in animals with AMI. The AMI induced changes in the LV, observed by the structural and functional variables of the echocardiogram. In the isolated heart analysis, the variables of positive pressure-time derivative (+dP/dtmax) and negative pressure-time derivative (– dP/dtmax) were lower between the AMI groups, while the area under the curve (AUC) was elevated in animals with AMI. The myocyte cross-sectional area (AST) was higher in the AMI groups, under oxidative stress, it was not possible to observe a difference in total protein carbonylation, in addition, there was no difference in the expressions of NRF-2 and collagen III proteins. Conclusion: bean flour supplementation did not attenuate the CR induced by AMI in rats.
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Palavras-chave
Infarto do miocárdio, Remodelação ventricular, Estresse oxidativo, Compostos fenólicos, Phaseolus vulgaris, Myocardial infarction, Ventricular remodeling, Oxidative stress