Avaliação do reparo ósseo em defeitos críticos preenchidos com biocerâmicas sintéticas e uma membrana sintética à base de polidioxanona.
Carregando...
Data
2022-03-04
Autores
Almeida, Lilian Caldas Quirino de
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Estadual Paulista (Unesp)
Resumo
O uso de materiais regenerativos é uma das técnicas indicadas para a reconstrução de defeitos ósseos, com resultados mais eficazes e previsíveis, graças ao desenvolvimento da bioengenharia. O objetivo deste trabalho foi avaliar o processo de reparo ósseo utilizando biocerâmicas sintéticas e membrana absorvível a base de polidioxanona (PDO) em defeitos ósseos críticos, realizados em calvária de ratos. Os resultados foram formatados em 3 artigos científicos. Sendo, que os dois primeiros estão relacionados às biocerâmicas e o terceiro artigo, com a regeneração óssea guiada por meio da membrana de polidioxanona. Em todos os artigos foram realizadas análises de histometria, microtomografia computadorizada, imunoistoquímica e PCR. Neste estudo foram utilizados 185 animais, com a confecção de dois defeitos críticos por animal, divididos em 10 grupos experimentais, preenchidos da seguinte forma: G1: Plenum® Oss HA (PHA), G2: coágulo sanguíneo (CG); G3: Plenum® Oss β-TCP, G4 (PB): Cerasorb® (CS) G5: Plenum® Oss hp (PO), G6: BoneCeramic® (BC), G7: Plenum® Oss hp + Plenum® Guide (PG), G8: Plenum® Guide + coágulo (PC), e G9: Bio-Gide® + Plenum® Oss (BG) hp e G10: Bio-Gide® + coágulo (BC). Os animais foram submetidos a eutanásia após os períodos de 07, 15, 30 e 60 dias. As amostras foram coletadas e preparadas para todas as análises propostas. Na comparação entre os grupos PO e BC, não observamos diferenças estatisticamente significantes entre a formação de tecido conjuntivo, tecido ósseo e os remanescentes do biomaterial entre os grupos. Na comparação entre os grupos PB e CS, dentro dos parâmetros avaliados, não houve diferença estatística entre si, contudo, as condições celulares iniciais foram mais favoráveis para o grupo PB, ao avaliar às análises de biologia molecular. Quando comparados os grupos PG e BG, não observamos diferenças significativas pelas análises de histometria e microtomografia computadorizada, porém, o grupo PG apresentou uma imunomarcação melhor em relação ao grupo BG. Concluímos que os materiais testados pelas análises propostas neste estudo apresentaram resultados satisfatórios e não apresentaram diferenças em comparação aos demais materiais comparadores, sendo, portanto, considerados uma opção viável como substituto ósseo sintético, bem como barreira na regeneração óssea guiada.
The use of regenerative materials is one of the techniques indicated for the repair/reconstruction of bone defects, with more effective and predictable results, thanks to the development of bioengineering. The objective of this work was to evaluate the bone repair process using synthetic bioceramics and a polydioxanone (PDO)-based resorbable membrane in critical bone defects in rat calvaria. The results were formatted in 3 scientific articles. The first two are related to bioceramics and the third article, with bone regeneration guided by a polydioxanone membrane. Histometry, computed microtomography, immunohistochemistry, and PCR analyzes were performed in all three articles. 185 rats were used in this study, with two critical defects per animal, divided into 10 experimental groups as follows: G1: Plenum® Oss HA (PHA), G2: blood clot (CG); G3: Plenum® Oss β-TCP, G4 (PB): Cerasorb® (CS) G5: Plenum® Oss hp (PO), G6: BoneCeramic® (BC), G7: Plenum® Oss hp + Plenum® Guide (PG), G8: Plenum® Guide + clot (PC), and G9: Bio-Gide® + Plenum® Oss (BG) hp and G10: Bio-Gide® + clot (BC). The animals were euthanized after periods of 07, 15, 30, and 60 days. The samples were collected and prepared for all proposed analyses. Comparing the PO and BC groups, we did not observe statistically significant differences between the formation of connective tissue, bone tissue, and biomaterial remnants amongst the groups by the proposed analyses. In the comparison between the PB and CS groups, within the evaluated parameters, there were no statistical differences between them, however, the initial cellular conditions were more favorable for the PB group by the molecular biology analyses. When comparing the PG and BG groups, we did not observe significant differences by histometry and computed microtomography analyses, however, the PG group presented better immunostaining in relation to the BG group. We conclude that the materials tested by the analyzes proposed in this study presented satisfactory results and did not present differences compared to the other control materials, therefore, considered viable options as synthetic bone substitutes, as well as a barrier in guided bone regeneration.
The use of regenerative materials is one of the techniques indicated for the repair/reconstruction of bone defects, with more effective and predictable results, thanks to the development of bioengineering. The objective of this work was to evaluate the bone repair process using synthetic bioceramics and a polydioxanone (PDO)-based resorbable membrane in critical bone defects in rat calvaria. The results were formatted in 3 scientific articles. The first two are related to bioceramics and the third article, with bone regeneration guided by a polydioxanone membrane. Histometry, computed microtomography, immunohistochemistry, and PCR analyzes were performed in all three articles. 185 rats were used in this study, with two critical defects per animal, divided into 10 experimental groups as follows: G1: Plenum® Oss HA (PHA), G2: blood clot (CG); G3: Plenum® Oss β-TCP, G4 (PB): Cerasorb® (CS) G5: Plenum® Oss hp (PO), G6: BoneCeramic® (BC), G7: Plenum® Oss hp + Plenum® Guide (PG), G8: Plenum® Guide + clot (PC), and G9: Bio-Gide® + Plenum® Oss (BG) hp and G10: Bio-Gide® + clot (BC). The animals were euthanized after periods of 07, 15, 30, and 60 days. The samples were collected and prepared for all proposed analyses. Comparing the PO and BC groups, we did not observe statistically significant differences between the formation of connective tissue, bone tissue, and biomaterial remnants amongst the groups by the proposed analyses. In the comparison between the PB and CS groups, within the evaluated parameters, there were no statistical differences between them, however, the initial cellular conditions were more favorable for the PB group by the molecular biology analyses. When comparing the PG and BG groups, we did not observe significant differences by histometry and computed microtomography analyses, however, the PG group presented better immunostaining in relation to the BG group. We conclude that the materials tested by the analyzes proposed in this study presented satisfactory results and did not present differences compared to the other control materials, therefore, considered viable options as synthetic bone substitutes, as well as a barrier in guided bone regeneration.
Descrição
Palavras-chave
Substitutos ósseos, Polidioxanona, Materiais biomédicos, Bone substitutes, Polydioxanone, Biomedical materials