Crystallization and preliminary X-ray diffraction analysis of a class II phospholipase D from Loxosceles intermedia venom
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Data
2011-02-01
Autores
Ullah, Anwar [UNESP]
de Giuseppe, Priscila Oliveira
Murakami, Mario Tyago
Trevisan-Silva, Dilza
Martins Wille, Ana Carolina
Chaves-Moreira, Daniele
Gremski, Luiza Helena
da Silveira, Rafael Bertoni
Sennf-Ribeiro, Andrea
Chaim, Olga Meiri
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Wiley-Blackwell
Resumo
Phospholipases D are the major dermonecrotic component of Loxosceles venom and catalyze the hydrolysis of phospholipids, resulting in the formation of lipid mediators such as ceramide-1-phosphate and lysophosphatidic acid which can induce pathological and biological responses. Phospholipases D can be classified into two classes depending on their catalytic efficiency and the presence of an additional disulfide bridge. In this work, both wild-type and H12A-mutant forms of the class II phospholipase D from L. intermedia venom were crystallized. Wild-type and H12A-mutant crystals were grown under very similar conditions using PEG 200 as a precipitant and belonged to space group P12(1)1, with unit-cell parameters a = 50.1, b = 49.5, c = 56.5 angstrom, beta = 105.9 degrees. Wild-type and H12A-mutant crystals diffracted to maximum resolutions of 1.95 and 1.60 angstrom, respectively.
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Acta Crystallographica Section F-structural Biology and Crystallization Communications. Malden: Wiley-blackwell, v. 67, p. 234-236, 2011.