Quantitative determination of maitenin and 22β-hydroxymaitenin in callus of Maytenus aquifolium (Celastraceae) by reverse phase high performance liquid chromatography
Nenhuma Miniatura disponível
Data
1998-01-01
Autores
Corsino, Joaquim [UNESP]
Alécio, Alberto Camilo [UNESP]
Ribeiro, Maria Lücia [UNESP]
Furlan, Maysa [UNESP]
Pereira, Ana Maria S.
Duarte, Ines Batista
França, Suzelei Castro
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
Explants of Maytenus aquifolium were induced to form callus and, subsequently, suspension cultures. The isolation of natural products from callus led to the identification of the cytotoxic triterpene quinonemethides, maitenin (1) and 22β-hydroxymaitenin (2). A rapid, sensitive and reliable reversed-phase high-performance liquid chromatography method was developed using a C18 column and isocratic elution for the determination of 1 and 2. The elaborated method gave well-resolved peaks for these compounds with good detection response and linearity in the range of 0.08-72.0 μg. The quantification of 1 and 2 was performed by an external standard method.
Descrição
Palavras-chave
22β-hydroxymaitenin, Callus, Cell culture, Maitenin, Maytenus aquifolium, Quantitative reversed-phase high-performance liquid chromatography
Como citar
Phytochemical Analysis, v. 9, n. 5, p. 245-247, 1998.