Identification of Salmonella spp. isolates from chicken abattoirs by multiplex-PCR
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Data
2006-12-01
Autores
Cortez, A. L. L.
Carvalho, A. C. F. B.
Ikuno, A. A.
Burger, K. P.
Vidal-Martins, A. M. C.
Título da Revista
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Editor
W B Saunders Co Ltd
Resumo
The present study was carried out to report the occurrence Salmonella spp., Salmonella Enteritidis, and Salmonella Typhimurium in chicken abattoirs. Samples of feces; feathers; scald, evisceration, and chiller water; and rinse water of non-eviscerated, eviscerated, and chilled carcass were collected from six chicken abattoirs. Salmonella isolates were identified by a multiplex-PCR using three sets of primers targeting the inuA, pefA, and sefA gene sequences from Salmonella spp., S. Typhimurium and S. Enteritidis, respectively. Salmonella spp. was detected in 10% (29/288) of the samples, whereas serovars Enteritidis and Typhimurium were identified in 62% (7/288), respectively. The results indicate the need to improve hygiene and sanitary standards in poultry slaughter lines, besides the education of food handlers and information to consumers. (c) 2006 Elsevier Ltd. All rights reserved.
Descrição
Palavras-chave
Salmonella spp., Salmonella enteritidis, Salmonella typhimurium, multiplex-PCR, chicken abattoir
Como citar
Research In Veterinary Science. London: W B Saunders Co Ltd, v. 81, n. 3, p. 340-344, 2006.