Iron chelating-mediated antioxidant activity of Plectranthus barbatus extract on mitochondria
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Data
2010-09-01
Autores
Maioli, Marcos A. [UNESP]
Alves, Larissa C. [UNESP]
Campanini, Andre L. [UNESP]
Lima, Michele C. [UNESP]
Dorta, Daniel J.
Groppo, Milton
Cavalheiro, Alberto José [UNESP]
Curti, Carlos
Mingatto, Fábio Erminio [UNESP]
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Editor
Elsevier B.V.
Resumo
Plectranthus barbatus Andrews (Lamiaceae) is a popular medicinal plant used to treat gastrointestinal and hepatic ailments. In this work, we assessed the antioxidant activity of the aqueous extract of P. barbatus leaves on Fe(2+)-citrate-mediated membrane lipid peroxidation in isolated rat liver mitochondria, as well in non-mitochondrial systems: DPPH reduction, (center dot)OH scavenging activity, and iron chelation by prevention of formation of the Fe(2+)-bathophenanthroline disulfonic acid (BPS) complex. Within all the tested concentrations (15-75 mu g/ml), P. barbatus extract presented significant free radical-scavenging activity (IC(50) = 35.8 +/- 0.27 mu g/ml in the DPPH: assay and IC(50) = 69.1 +/- 0.73 mu g/ml in the (center dot)OH assay) and chelated iron (IC(50) = 30.4 +/- 3.31 mu g/ml). Over the same concentration range, the plant extract protected mitochondria against Fe(2+)/citrate-mediated swelling and malondialdehyde production, a property that persisted even after simulation of its passage through the digestive tract. These effects could be attributed to the phenolic compounds, nepetoidin - caffeic acid esters, present in the extract. Therefore, P. barbatus extract prevents mitochondrial membrane lipid peroxidation, probably by chelation of iron, revealing potential applicability as a therapeutic source of molecules against diseases involving mitochondrial iron overload. (C) 2010 Elsevier Ltd. All rights reserved.
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Palavras-chave
Plectranthus barbatus, Mitochondria, Reactive oxygen species, Lipid peroxidation, Antioxidant, Iron chelation
Como citar
Food Chemistry. Oxford: Elsevier B.V., v. 122, n. 1, p. 203-208, 2010.