Determinação simultânea de resíduos de cloranfenicol, tianfenicol e florfenicol em leite bovino por cromatografia eletrocinética micelar
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Data
2006-10-01
Autores
Pezza, Leonardo [UNESP]
Ríos, Àngel
Nozal, Leonor
Arce, Lourdes
Valcárce, Miguel
Título da Revista
ISSN da Revista
Título de Volume
Editor
Sociedade Brasileira de Química
Resumo
A micellar electrokinetic chromatographic method (MEKC) is described for determining residues of amphenicols(chloramphenicol,thiamphenicol and florfenicol) in bovine milk. MEKC is conducted by using a separation buffer consisting of 20 mM Na2HPO4, 10 mM Na2B4O7, 50 mM SDS at pH 8.0; UV detection at 210 nm and 10 kV of voltage. The limit of detection ranged from 4.3-5.3 µg L-1. The MEKC method was applied for the simultaneous determination of amphenicols in milk samples spiked with amphenicols at three concentration levels: 10, 30 and 50 µg L-1. Recoveries ranging from 91-105% were obtained by following a simple extraction/preconcentration procedure.
Descrição
Palavras-chave
micelar electrokinetic chromatography, amphenicols, milk
Como citar
Química Nova. Sociedade Brasileira de Química, v. 29, n. 5, p. 926-931, 2006.