Preparative separation of flavonoids from the medicinal plant Davilla elliptica St. Hill. by high-speed counter-current chromatography
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Data
2006-10-01
Autores
Rinaldo, Daniel [UNESP]
Silva, Marcelo Aparecido [UNESP]
Rodrigues, Clenilson Martins [UNESP]
Calvo, Tamara Regina [UNESP]
Sannomiya, Miriam [UNESP]
Santos, Lourdes Campaner dos [UNESP]
Vilegas, Wagner [UNESP]
Kushima, Hélio [UNESP]
Hiruma-Lima, Clélia Akiko [UNESP]
Brito, Alba Regina Monteiro de Souza
Título da Revista
ISSN da Revista
Título de Volume
Editor
Sociedade Brasileira de Química
Resumo
High-speed counter-current chromatography (HSCCC) is a major tool for the fast separation of natural products from plants. It was used for the preparative isolation of the flavonoid monoglucosides present in the aerial parts of the Davilla elliptica St. Hill. (Dilleniaceae). This species is used in Brazilian folk medicine for the treatment of gastric disorders. The optimum solvent system used was composed of a mixture of ethyl acetate-n-propanol-water (140:8:80, v/v/v) and led to a successful separation of quercetin-3-O-alpha-L-rhamnopyranoside and myricetin-3-O-alpha-L-rhamnopyranoside in approximately 3.0 hours with purity higher than 95%. Identification was performed by ¹H NMR, 13C NMR and HPLC-UV-DAD analyses.
Descrição
Palavras-chave
HSCCC, Davilla elliptica, flavonoids
Como citar
Química Nova. Sociedade Brasileira de Química, v. 29, n. 5, p. 947-949, 2006.