Identificação de alguns constituintes químicos de Indigofera hirsuta Linn. (Fabaceae) por CLAE-IES-EM (TOF) e avaliação da atividade antirradicalar
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Data
2011-01-01
Autores
Moura, Adriana Candido da Silva [UNESP]
Vilega, Wagner [UNESP]
Santos, Lourdes Campaner dos [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Sociedade Brasileira de Química
Resumo
A rapid analytical approach, suitable to characterize the compounds present in the aqueous and methanol extracts prepared from the aerial parts of Indigofera hirsute, was developed. The method based on high-performance liquid chromatography coupled to mass spectrometry, electrospray positive ionization and detection by time of flight (HPLC-ESI-MS-TOF) identified, tryptophan, uracil, rutin, kaempferol-3-O-β-D-glucopyranoside, gallic acid and methyl gallate. The antiradical activity of this extract was evaluated using DPPH assay, with gallic acid as antiradical pattern. The study revealed the antiradical activity of methyl galatte (EC50 = 5 ± 0.3 µg mL-1) galic acid (EC50 = 5 ± 0.2 µg mL-1) and rutin (EC50 = 21.6 ± 0.6 µg m L-1), isolated from methanol extract (EC50 = 67.7 ± 0.9 µg mL-1), which showed strong antiradical activity.
Descrição
Palavras-chave
Indigofera hirsuta, mass spectrometry, antiradical activity
Como citar
Química Nova. Sociedade Brasileira de Química, v. 34, n. 7, p. 1136-1140, 2011.