Reimplante mediato de dentes tratados ou não com solução de hipoclorito de sódio a 1% - estudo histomorfométrico em ratos
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Data
2000-06-01
Autores
KANNO, Cláudia Misue
SAAD NETO, Michel [UNESP]
SUNDFELD, Maria Lúcia Marçal Mazza [UNESP]
VELASCO-BOHÓRQUES, Marina del Pilar [UNESP]
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Editor
Sociedade Brasileira de Pesquisa Odontológica e Faculdade de Odontologia da Universidade de São Paulo
Resumo
Foi avaliada a influência do hipoclorito de sódio a 1% no reparo do periodonto de incisivos reimplantados tardiamente em ratos. Os dentes foram mantidos em meio ambiente durante 30 minutos e a seguir imersos em hipoclorito de sódio a 1%, por 25 minutos. Os animais foram sacrificados aos 10, 30 ou 60 dias. Os cortes histológicos foram obtidos no sentido transversal dos terços cervical, médio e apical e delineados com um aumento de 52 vezes. Os eventos observados sobre a superfície radicular foram medidos quanto a sua extensão através de um sistema analisador de imagens (Mini-Mop, Carl Zeiss). Aos 10 dias, observou-se a predominância de tecido conjuntivo fibroso no grupo tratado. Aos 30 e 60 dias observou-se o aumento da anquilose e reabsorção por substituição, assim como neoformação limitada de ligamento periodontal. No grupo controle, aos 30 e 60 dias, foram observadas áreas de reabsorções mais extensas e profundas, quando comparadas ao grupo tratado. Os resultados possibilitaram as seguintes conclusões quando comparado o grupo tratado com o controle: a) leva à formação de tecido conjuntivo semelhante a uma cápsula fibrosa no período inicial do reparo; b) possibilita uma limitada neoformação de ligamento periodontal; c) ocasiona uma superfície de anquilose mais extensa; d) não impede a reabsorção por substituição.
The aim of the present study was to examine the influence of 1% sodium hypochlorite on periodontal healing after tooth replantation. Rats incisors were extracted, maintained in the environment during 30 minutes, then immersed in 1% sodium hypochlorite for 30 minutes. In the control group, the teeth were maintained in distilled water. The animals were sacrificed after 10, 30 and 60 days. Transversal slices of the teeth were obtained and histologically processed through the hematoxylin and eosin technique. Slices from the cervical, middle and apical thirds of each tooth were selected for the analysis. The slices were projected and outlined. The tissues observed on the dental surfaces were measured with an image analysing system (Mini-Mop, Carl Zeiss). In the 10-day treated group, the main characteristic observed was a connective tissue arranged parallelly to the root surface, although not attached to it. After 30 and 60 days, the connective tissue was reduced and an increase of ankylosis was observed, as well as replacement resorption. A limited periodontal ligament reattachment was also observed. In the control group, the periodontal ligament was preserved in about 15% to 17% of the root surface after 30 and 60 days, and the resorption areas were deeper than in the treated group. It was concluded that 1% sodium hypochlorite allowed an increase of the ankylosed root surface and a limited periodontal ligament reattachment, but did not avoid replacement resorption.
The aim of the present study was to examine the influence of 1% sodium hypochlorite on periodontal healing after tooth replantation. Rats incisors were extracted, maintained in the environment during 30 minutes, then immersed in 1% sodium hypochlorite for 30 minutes. In the control group, the teeth were maintained in distilled water. The animals were sacrificed after 10, 30 and 60 days. Transversal slices of the teeth were obtained and histologically processed through the hematoxylin and eosin technique. Slices from the cervical, middle and apical thirds of each tooth were selected for the analysis. The slices were projected and outlined. The tissues observed on the dental surfaces were measured with an image analysing system (Mini-Mop, Carl Zeiss). In the 10-day treated group, the main characteristic observed was a connective tissue arranged parallelly to the root surface, although not attached to it. After 30 and 60 days, the connective tissue was reduced and an increase of ankylosis was observed, as well as replacement resorption. A limited periodontal ligament reattachment was also observed. In the control group, the periodontal ligament was preserved in about 15% to 17% of the root surface after 30 and 60 days, and the resorption areas were deeper than in the treated group. It was concluded that 1% sodium hypochlorite allowed an increase of the ankylosed root surface and a limited periodontal ligament reattachment, but did not avoid replacement resorption.
Descrição
Palavras-chave
Reimplante dental, Hipoclorito de sódio, Reabsorção da raiz, Tooth replantation, Sodium hypochlorite, Root resorption
Como citar
Pesquisa Odontológica Brasileira. Sociedade Brasileira de Pesquisa Odontológica e Faculdade de Odontologia da Universidade de São Paulo, v. 14, n. 2, p. 151-157, 2000.