Histological study of the effect of some irrigating solutions on bacterial endotoxin in dogs
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Data
2004-01-01
Autores
Silva, Léa Assed Bezerra da
Leonardo, Mario Roberto [UNESP]
Assed, Sada
Tanomaru Filho, Mario [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Fundação Odontológica de Ribeirão Preto
Resumo
O objetivo deste estudo foi avaliar, histopatologicamente, o efeito do preparo biomecânico com diferentes soluções irrigadoras em dentes de cães preenchidos com LPS bacteriano após pulpectomia. Foram utilizados 120 canais radiculares de 6 cães, os quais foram preenchidos com solução de LPS após pulpectomia. As soluções irrigadoras usadas foram solução de hipoclorito de sódio a 1, 2.5 e 5% e solução de clorexidina a 2%. Nenhuma irrigação foi realizada no grupo controle. Os animais foram mortos após 60 dias, e os dentes foram fixados e desmineralizados. em seguida, cortes seriados de 6 µm de espessura foram corados com hematoxilina e eosina e Tricrômico de Mallory para análise histopatológica e Brown e Brenn para verificação da contaminação bacteriana. A avaliação demonstrou que o infiltrado inflamatório foi estatisticamente menos intenso nos grupos cujos canais radiculares foram irrigados com solução de hipoclorito de sódio a 5% e de clorexidina a 2%. Contudo, nenhuma das soluções irrigadoras inativou completamente os efeitos deletérios do LPS. O preparo biomecânico coadjuvado por diferentes soluções irrigadoras não inativaram completamente a endotoxina.
The aim of this study was to evaluate, histopathologically, the effectiveness of mechanical preparation of root canals using different irrigating solutions in dog teeth filled with LPS after pulpectomy. A total of 120 root canals of 6 mongrel dogs were filled with a solution of LPS after pulpectomy. The irrigating solutions used were saline, 1, 2.5, and 5% sodium hypochlorite, and 2% chlorhexidine. No irrigation was used in the control group. The animals were sacrificed after 60 days and the teeth were fixed and demineralized. Subsequently, serial 6-µm sections were stained with hematoxylin and eosin and Mallory's trichrome for histopathological analysis and Brown-Brenn for verification of bacterial contamination. Analysis showed that the inflammatory infiltrate was statistically less intense in the groups in which the root canals were irrigated with 5% sodium hypochlorite and 2% chlorhexidine. However, none of the irrigating solutions completely inactivated the harmful effects of LPS. Mechanical preparation associated with different irrigating solutions did not completely inactivate LPS.
The aim of this study was to evaluate, histopathologically, the effectiveness of mechanical preparation of root canals using different irrigating solutions in dog teeth filled with LPS after pulpectomy. A total of 120 root canals of 6 mongrel dogs were filled with a solution of LPS after pulpectomy. The irrigating solutions used were saline, 1, 2.5, and 5% sodium hypochlorite, and 2% chlorhexidine. No irrigation was used in the control group. The animals were sacrificed after 60 days and the teeth were fixed and demineralized. Subsequently, serial 6-µm sections were stained with hematoxylin and eosin and Mallory's trichrome for histopathological analysis and Brown-Brenn for verification of bacterial contamination. Analysis showed that the inflammatory infiltrate was statistically less intense in the groups in which the root canals were irrigated with 5% sodium hypochlorite and 2% chlorhexidine. However, none of the irrigating solutions completely inactivated the harmful effects of LPS. Mechanical preparation associated with different irrigating solutions did not completely inactivate LPS.
Descrição
Palavras-chave
endotoxin, root canal therapy, irrigating solution
Como citar
Brazilian Dental Journal. Fundação Odontológica de Ribeirão Preto, v. 15, n. 2, p. 109-114, 2004.