Análise morfológica dos ovários de fetos bovinos da raça Nelore (Bos primigenius indicus) em diferentes fases de gestação
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Data
2003-01-01
Autores
Monteiro, Cristina Maria Rodrigues [UNESP]
Carvalhal, Roberto [UNESP]
Perri, Silvia Helena Venturoli [UNESP]
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Editor
Universidade de São Paulo (USP), Faculdade de Medicina Veterinária e Zootecnia (FMVZ)
Resumo
Vinte fetos fêmeas em diferentes fases de gestação da raça Nelore foram coletados para a realização desta pesquisa. Os ovários direitos e esquerdos foram dissecados e mensurados para verificação de seus comprimentos e larguras e em seguida, foram fixados inteiros em paraformaldeído tamponado a 4,00%, processados e incluídos em paraplástico. Os cortes com 5 mm foram submetidos à coloração com hematoxilina, tricrômio de Masson (para fibras colágenas), Verhoeff (para fibras elásticas) e com reticulina (para fibras reticulares). Os resultados mostraram que não há diferença significativa das mensurações entre os lados direito e esquerdo para os ovários dos fetos bovinos em diferentes fases de gestação, mas há correlação entre os valores obtidos das mensurações dos ovários em função da idade dos fetos, ou seja, o crescimento dos ovários acompanha o crescimento fetal. Os fetos em diferentes fases de gestação, apresentam epitélio germinativo, túnica albugínea e tecido conjuntivo no córtex e medula característicos da morfologia ovariana. Nos ovários de fetos com até 19 semanas de idade gestacional, é evidente a presença em grande quantidade de folículos primordiais e primários. A partir de 22 semanas de gestação, é evidente a presença em grande número, de folículos ovarianos em diferentes estágios de crescimento. A partir de 17 semanas de gestação, observa-se na medula, cordões com luz irregular revestidos por células cúbicas. As variações mais marcantes ocorreram a partir de 22 semanas de gestação.
In this research, twenty Nelore female fetuses in different phases of gestation were collected. The right and left ovaries were dissected and measured for verification of the length and width and soon after that, the entire ovaries were fixed in 4.00% tamponed paraformaldeido, processed and enclosed in paraplastic. The histological sections with 5 mm were stained with hematoxilin and eosin, Masson's trichrome (to colagens fibers), Verhoeff (to elastic fibers) and with reticulin (to reticulares fibers). The results showed that there is no significative differences among the right and left sides for the bovine ovaries in fetuses in different phases of gestation, but there is correlation of the ovaries values in function of the age of the fetuses, or either, the growth of the ovaries follow the fetal growth. The fetuses in different phases of gestation, present germinative epithelium, tunica albuginea and connective tissue in the cortical and medullar parts characteristically of ovarian morphology. In the ovaries of fetuses with up to 19 weeks of gestacional age, the presence in great amount of primordial and primary follicles is evident. From 22 weeks of gestation, the presence in great number, of follicles in different periods of growth is evident. From 17 weeks of gestation, was observed medullar cords with irregular lumen covered by cubical cells. The more characteristic variations occurred from 22 weeks of gestation.
In this research, twenty Nelore female fetuses in different phases of gestation were collected. The right and left ovaries were dissected and measured for verification of the length and width and soon after that, the entire ovaries were fixed in 4.00% tamponed paraformaldeido, processed and enclosed in paraplastic. The histological sections with 5 mm were stained with hematoxilin and eosin, Masson's trichrome (to colagens fibers), Verhoeff (to elastic fibers) and with reticulin (to reticulares fibers). The results showed that there is no significative differences among the right and left sides for the bovine ovaries in fetuses in different phases of gestation, but there is correlation of the ovaries values in function of the age of the fetuses, or either, the growth of the ovaries follow the fetal growth. The fetuses in different phases of gestation, present germinative epithelium, tunica albuginea and connective tissue in the cortical and medullar parts characteristically of ovarian morphology. In the ovaries of fetuses with up to 19 weeks of gestacional age, the presence in great amount of primordial and primary follicles is evident. From 22 weeks of gestation, the presence in great number, of follicles in different periods of growth is evident. From 17 weeks of gestation, was observed medullar cords with irregular lumen covered by cubical cells. The more characteristic variations occurred from 22 weeks of gestation.
Descrição
Palavras-chave
Morfologia, Ovários, Fetos, Bovino, Morphology, Ovaries, Fetuses, Bovine
Como citar
Brazilian Journal of Veterinary Research and Animal Science. Faculdade de Medicina Veterinária e Zootecnia / Universidade de São Paulo, v. 40, n. 6, p. 409-415, 2003.