RAPID COAGGLUTINATION TEST FOR THE DETECTION AND TYPING OF FOOT-AND-MOUTH-DISEASE VIRUS
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Data
1994-12-01
Autores
Montassier, H. J.
Araujo, J. P.
Pinto, A. A.
Título da Revista
ISSN da Revista
Título de Volume
Editor
Elsevier B.V.
Resumo
Protein A containing Staphylococcus aureus was used to develop a coagglutination (COA) test for the detection and typing of foot and mouth disease virus (FMDV) O, A and C serotypes in infected cells and tissues. Different batches and amounts of guinea pig anti-FMDV sera were assessed to optimize the preparation of COA conjugates. The sensitivity and specificity of the COA Test for the detection of FMDV O, A and C serotypes and heterologous viruses was also characterized. Comparison between the COA Test and complement fixation test for the detection and typing of FMDV obtained from extracts of tongue epithelial tissues from infected cattle revealed high agreement in the results and indicated a potential application of the COA Test for the direct diagnosis of viruses.
Descrição
Palavras-chave
STAPHYLOCOCCAL COAGGLUTINATION, foot-and-mouth disease virus, RAPID DIAGNOSIS
Como citar
Journal of Virological Methods. Amsterdam: Elsevier B.V., v. 50, n. 1-3, p. 29-41, 1994.