Use of chemically modified silica with beta-diketoamine groups for separation of alpha-lactoalbumin from bovine milk whey by affinity chromatography
Nenhuma Miniatura disponível
Data
1997-01-15
Autores
Gambero, A.
Kubota, L. T.
Gushikem, Y.
Airoldi, C.
Granjeiro, J. M.
Taga, E. M.
Alcantara, EFC
Título da Revista
ISSN da Revista
Título de Volume
Editor
Academic Press Inc. Jnl-comp Subscriptions
Resumo
Silica gel surface was chemically modified with beta-diketoamine groups by reacting the silanol from the silica surface with 3-aminopropyl-triethoxysilane and 3-bromopentanedione, With this material, copper ions were adsorbed from aqueous solutions, the chemical analysis of the silica-gel-immobilized acetylacetone provided a quantity of 0.67 mmol g(-1) of organic groups attached to the support and 0.63 mmol g(-1) of copper, This material was used as a stationary phase in IMAC (immobilized metal affinity chromatography), to separate alpha-lactoalbumin from bovine milk whey, the results showed an efficient separation in the chromatographic column, the possibility of reutilization of the stationary phase was also investigated. (C) 1997 Academic Press
Descrição
Palavras-chave
modified silica, affinity chromatography, separation, lactoalbumin
Como citar
Journal of Colloid and Interface Science. San Diego: Academic Press Inc. Jnl-comp Subscriptions, v. 185, n. 2, p. 313-316, 1997.