Type 2 heat-labile enterotoxin (LT-II)-producing Escherichia coli isolated from ostriches with diarrhea
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Data
2005-02-25
Autores
Nardi, ARM
Salvadori, M. R.
Coswig, L. T.
Gatti, MSV
Leite, D. S.
Valadares, G. E.
Neto, M. G.
Shocken-Iturrino, R. P.
Blanco, J. E.
Yano, T.
Título da Revista
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Editor
Elsevier B.V.
Resumo
The culture supernatant of Escherichia coli, isolated from ostriches with diarrhea in Brazil, caused elongation in Vero cell, rounding in Chinese hamster ovary (CHO) cells and a cytoplasmic vacuolation in ostrich embryo fibroblasts (OEF), but it was not cytotoxic for chicken embryo fibroblasts (CEF). These effects were not neutralized by antiserum to cholera toxin. Polymerase chain reaction assays showed that the ostrich E.coli contained the gene encoding (eltII-A), but not those for type 1 heat-labile enterotoxin (eltA), heat-stable enterotoxins (estA, estB), verocytotoxins (stx-I, stx-II), or cytotoxic necrotizing factors (cnf 1, cnf 2). All isolates belonged to serotype O15:H8. The enteropathogenic relevance of LT-II in ostrich diarrhea remains undetermined. (C) 2004 Elsevier B.V. All rights reserved.
Descrição
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Escherichia coli, type 2 heat-labile enterotoxin (LT-II), ostrich, diarrhea, cytoplasmic vacuolation
Como citar
Veterinary Microbiology. Amsterdam: Elsevier B.V., v. 105, n. 3-4, p. 245-249, 2005.