Micropropagation of fig (Ficus carica L.) 'Roxo de Valinhos' plants
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Data
2004-09-01
Autores
Fraguas, C. B.
Pasqual, M.
Dutra, L. F.
Cazetta, J. O.
Título da Revista
ISSN da Revista
Título de Volume
Editor
C A B I Publishing
Resumo
An in vitro protocol for Ficus carica cv. 'Roxo de Valinhos' was optimized. Nodal explants containing two buds were excised from field-grown mature plants, and transferred to different proliferation media consisting of combinations of distinct concentrations of activated charcoal with benzyladenine (BA), kinetin with gibberellic acid (GA(3)), and WPM (woody plant medium) with kinetin. The regular strength of WPM in combination with 0.5 mg l(-1) kinetin was the best condition for shoot proliferation of Ficus carica 'Roxo de Valinhos' plants. The addition of activated charcoal in the medium completely inhibited shoot proliferation. The inclusion of BA in the medium induced excessive callus formation as well as small and vitrified shoots, while GA(3) induced excessive elongation associated with vitrification, chlorosis, and tip-burned shoots.
Descrição
Palavras-chave
activated charcoal, growth regulators, shoot proliferation, tissue culture
Como citar
In Vitro Cellular & Developmental Biology-plant. Wallingford: C A B I Publishing, v. 40, n. 5, p. 471-474, 2004.