Araçatuba virus: A vaccinialike virus associated with infection in humans and cattle
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Data
2003-02-01
Autores
De Souza Trindade, Giliane
Da Fonseca, Flávio Guimarães
Marques, João Trindade
Nogueira, Maurício Lacerda
Mendes, Luiz Claudio Nogueira [UNESP]
Borges, Alexandre Secorun [UNESP]
Peiró, Juliana Regina [UNESP]
Pituco, Edviges Maristela
Bonjardim, Cláudio Antônio
Ferreira, Paulo César Peregrino
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Resumo
We describe a vaccinialike virus, Araçatuba virus, associated with a cowpoxlike outbreak in a dairy herd and a related case of human infection. Diagnosis was based on virus growth characteristics, electron microscopy, and molecular biology techniques. Molecular characterization of the virus was done by using polymerase chain reaction amplification, cloning, and DNA sequencing of conserved orthopoxvirus genes such as the vaccinia growth factor (VGF), thymidine kinase (TK), and hemagglutinin. We used VGF-homologous and TK gene nucleotide sequences to construct a phylogenetic tree for comparison with other poxviruses. Gene sequences showed 99% homology with vaccinia virus genes and were clustered together with the isolated virus in the phylogenetic tree. Araçatuba virus is very similar to Cantagalo virus, showing the same signature deletion in the gene. Araçatuba virus could be a novel vaccinialike virus or could represent the spread of Cantagalo virus.
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Palavras-chave
animal cell, Aracatuba virus, cattle disease, controlled study, cowpox, DNA sequence, DNA virus, gene amplification, gene cluster, gene deletion, gene sequence, molecular biology, molecular cloning, nonhuman, nucleotide sequence, Orthopoxvirus, phylogeny, polymerase chain reaction, sequence analysis, sequence homology, Vaccinia virus, Vero cell, virogenesis, virus gene, virus identification, virus infection, virus isolation, Animals, Base Sequence, Cattle, Cattle Diseases, Communicable Diseases, Emerging, Genes, Viral, Humans, Microscopy, Electron, Molecular Sequence Data, Phylogeny, Sequence Alignment, Vaccinia, Viral Proteins, Viral Structural Proteins, Viremia
Como citar
Emerging Infectious Diseases, v. 9, n. 2, p. 155-160, 2003.