Estrutura genética de populações de Trichilia pallida Swartz (Meliaceae) por marcadores RAPD
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Data
2004-06-01
Autores
Zimback, Léo
Mori, Edson Seizo [UNESP]
Kageyama, Paulo Yoshio
De Arruda Veiga, Renato Ferraz
Mello Jr., José Roberta Silveira [UNESP]
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Resumo
Trichilia pallida Swartz is an Atlantic Forest shady climax tree of Meliaceae family that presents insecticide properties against chewing insects like as some family trees, making it interesting for forestry uses. Forty plants of Oito Pontas Farm population were collected in Bofete County, Santa Genebra Ecological Station in Campinas County, and Caetetus Ecological Station in Gélia County, all in the Sao Paulo State, Brazil. Leaf DNA analysis was used by RAPD method, that showed 10 highly polymorphic primers, with 72 dominant markers, used to estimate genetic diversity within and among populations. The polymorphism within populations varied from 90.3 to 97.2%, and the effective allele number varied from 1.46 ± 0.33 to 1.57 ± 0.33, while the average of genetic differentiation of populations varied from 0.27 ± 0.18 to 0, 33 ± 0.15. The gene diversity in the total population (H T) was 0.334 ± 0.02, while the average gene diversity within populations (H s) was 0.292 ± 0.017, and the coefficient of gene differentiation (G ST) was 0.125, Bofete and Campinas populations had the smallest Nei's genetic distance (0.049) and the distances of both with Gália were 0.117 and 0.107, respectively.
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Palavras-chave
Genetic distance, Genetic diversity, RAPD, Polymorphic primers, Biodiversity, DNA, Ecosystems, Genes, Insect control, Insecticides, Plants (botany), Forestry, Insects, Nucleic Acids, Trees
Como citar
Scientia Forestalis/Forest Sciences, n. 65, p. 114-119, 2004.