Role of annexin 1 gene expression in mouse craniofacial bone development
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Data
2007-07-01
Autores
Damazo, Amilcar Sabino
Moradi-Bidhendi, Niloufar
Oliani, Sonia Maria [UNESP]
Flower, Roderick John
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Resumo
BACKGROUND: Annexin 1 is a 37-kDa protein that has complex intra- and extracellular effects. To discover whether the absence of this protein alters bone development, we monitored this event in the annexin-A1 null mice in comparison with littermate wild-type controls. METHODS: Radiographic and densitometry methods were used for the assessment of bone in annexin-A1 null mice at a gross level. We used whole-skeleton staining, histological analysis, and Western blotting techniques to monitor changes at the tissue and cellular levels. RESULTS: There were no gross differences in the appendicular skeleton between the genotypes, but an anomalous development of the skull was observed in the annexin-A1 null mice. This was characterized in the newborn annexin-A1 null animals by a delayed intramembranous ossification of the skull, incomplete fusion of the interfrontal suture and palatine bone, and the presence of an abnormal suture structure. The annexin-A1 gene was shown to be active in osteocytes during this phase and COX-2 was abundantly expressed in cartilage and bone taken from annexin-A1 null mice. CONCLUSIONS: Expression of the annexin-A1 gene is important for the normal development of the skull in mice, possibly through the regulation of osteoblast differentiation and a secondary effect on the expression of components of the cPLA2-COX-2 system. © 2007 Wiley-Liss, Inc.
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Palavras-chave
Annexin 1, Bone, Cyclooxygenase-2, Cytosolic phospholipase A2, Osteoblast, annexin, annexin 1, cyclooxygenase 2, phospholipase A2, protein, unclassified drug, adolescent, animal tissue, annexin a1 gene, bone density, controlled study, craniofacial development, female, gene, gene activation, histology, immunohistochemistry, male, mouse, newborn, nonhuman, ossification, osteocyte, priority journal, protein expression, skull suture, Western blotting, Animals, Animals, Newborn, Annexin A1, Bone and Bones, Bone Density, Bone Development, Craniofacial Abnormalities, Cyclooxygenase 2, Female, Gene Expression, Homozygote, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Osteogenesis, Phospholipases A, Animalia, Mus
Como citar
Birth Defects Research Part A - Clinical and Molecular Teratology, v. 79, n. 7, p. 524-532, 2007.