Susceptibility of planktonic cultures of Streptococcus mutans to photodynamic therapy with a light-emitting diode
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Data
2010-10-01
Autores
da Costa, Anna Carolina Borges Pereira [UNESP]
Chibebe Junior, José [UNESP]
Pereira, Cristiane Aparecida [UNESP]
Machado, Ana Karina da Silva [UNESP]
Beltrame Junior, Milton
Junqueira, Juliana Campos [UNESP]
Jorge, Antonio Olavo Cardoso [UNESP]
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Resumo
The objective of this study was to evaluate the effect of photodynamic therapy with erythrosine and rose bengal using a light-emitting diode (LED) on planktonic cultures of S. mutans. Ten S. mutans strains, including nine clinical strains and one reference strain (ATCC 35688), were used. Suspensions containing 10 6 cells/mL were prepared for each strain and were tested under different experimental conditions: a) LED irradiation in the presence of rose bengal as a photosensitizer (RB+L+); b) LED irradiation in the presence of erythrosine as a photosensitizer (E+L+); c) LED irradiation only (P-L+); d) treatment with rose bengal only (RB+L-); e) treatment with erythrosine only (E+L-); and f) no LED irradiation or photosensitizer treatment, which served as a control group (P-L-). After treatment, the strains were seeded onto BHI agar for determination of the number of colony-forming units (CFU/mL). The results were submitted to analysis of variance and the Tukey test (p ≤ 0.05). The number of CFU/mL was significantly lower in the groups submitted to photodynamic therapy (RB+L+ and E+L+) compared to control (P-L-), with a reduction of 6.86 log 10 in the RB+L+ group and of 5.16 log 10 in the E+L+ group. Photodynamic therapy with rose bengal and erythrosine exerted an antimicrobial effect on all S. mutans strains studied.
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Palavras-chave
Erythrosine, Photochemotherapy, Rose bengal, Streptococcus mutans, erythrosine, photosensitizing agent, rose bengal, analysis of variance, bacterial load, biofilm, cell culture, dental caries, drug effect, isolation and purification, methodology, photochemotherapy, radiation exposure, time, Analysis of Variance, Bacterial Load, Biofilms, Cells, Cultured, Dental Caries, Photosensitizing Agents, Rose Bengal, Time Factors
Como citar
Brazilian Oral Research, v. 24, n. 4, p. 413-418, 2010.