Assessment of DNA damage induced by extracts, fractions and isolated compounds of Davilla nitida and Davilla elliptica (Dilleniaceae)
Nenhuma Miniatura disponível
Data
2010-09-30
Autores
Biso, Fabiana Izilda [UNESP]
Rodrigues, Clenilson Martins [UNESP]
Rinaldo, Daniel [UNESP]
dos Reis, Mariana Bisarro
Bernardi, Caroline Cristiane
Pelielo de Mattos, Jose Carlos
Caldeira-de-Araujo, Adriano
Vilegas, Wagner [UNESP]
de Syllos Colus, Ilce Mara
Varanda, Eliana Aparecida [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Elsevier B.V.
Resumo
Davilla nitida and Davilla elliptica (Dilleniaceae) are plants that occur predominantly in the cerrado region of South America. They are used in popular medicine to treat stomach diseases, diarrhea and swelling, particularly of the lymph nodes and testicles. Chemical investigation of these two plant species led to the identification of the compounds myricetin-3-O-alpha-L-rhamnoside (myricitrin), quercetin-3-O-alpha-rhamnoside (quercitrin), myricetin, quercetin and gallic acid derivatives in the leaves of D. nitida and D. elliptica. Therefore, it was concluded that the two species of Davilla possess qualitatively similar chemical profiles. In the present study, the mutagenic and genotoxic potential of these plants and of their isolated compounds was tested in the Salmonella typhimurium assay (Ames test) with strains TA100,TA98,TA102 and TA97a, in the micronucleus test with peripheral blood cells of mice treated in vivo, and in plasmid DNA to analyze DNA strand-breaks. In the assessment of mutagenic potential by the Ames test, extracts from both plant species and a D. nitida ethyl-acetate fraction induced positive responses. on the other hand, none of the extracts showed genotoxic activity in the mouse cells. In the presence of metal ion, D. nitida and D. elliptica aqueous and ethyl-acetate fractions, as well as their isolated compounds, induced single- and double-strand-breaks in plasmid DNA in a cell-free system. (C) 2010 Elsevier B.V. All rights reserved.
Descrição
Palavras-chave
Davilla nitida, Davilla elliptica, Flavonoids, Ames test, Micronucleus test, DNA strand-breaks
Como citar
Mutation Research-genetic Toxicology and Environmental Mutagenesis. Amsterdam: Elsevier B.V., v. 702, n. 1, p. 92-99, 2010.