Antioxidant and cytotoxic effects of crude extract, fractions and 4-nerolidylcathecol from aerial parts of pothomorphe umbellata L. (Piperaceae)

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2013-03-11

Autores

Lopes, Andrey P.
Bagatela, Bianca S.
Rosa, Paulo C. P.
Nanayakkara, Dhammika N. P.
Carlos Tavares Carvalho, José
Maistro, Edson Luis [UNESP]
Bastos, Jairo K.
Perazzo, Fábio F.

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Resumo

The crude ethanolic extract from aerial parts of Pothomorphe umbellata L. (Piperaceae) and fractions obtained by partitions sequentially among water-methanol, methylene chloride, and ethyl acetate, as well as the major constituent, 4-nerolidylcatechol, were, respectively, evaluated and evidenced for antioxidant and cytotoxic effects through fluorometric microplate and microculture tetrazolium assays in HL-60 cells. The crude ethanolic extract demonstrated the preeminent antioxidant activity (IC50 = 1.2 μg/mL) against exogenous cytoplasmic reactive oxygen species, followed by the water-methanolic (IC50 = 4.5 μg/mL), methylene chloride (IC 50 = 5.9 g/mL), ethyl acetate (IC50 = 8.0 g/mL), 4-nerolidylcatechol (IC50 = 8.6 g/mL), and the sterol fractions (IC50 > 12.5 μg/mL). Vitamin C, the positive control used in this assay, presented IC50 value equivalent to 1.7 μg/mL. 4-Nerolidylcatechol (IC50 = 0.4 μg/mL) and methylene chloride fraction (IC50 = 2.3 μg/mL) presented considerable cytotoxicity probably because of the presence of an o-quinone, an auto-oxidation by product of the catechol. Polar compounds, present in the ethanol extract, appear to increase the solubility and stability of the major active constituent, acting synergistically with 4-nerolidylcatechol, improving its pharmacokinetic parameters and increasing significantly its antioxidant activity which, in turn, suggests that the aqueous-ethanolic extract, used in folklore medicine, is safe and effective. © 2013 Andrey P. Lopes et al.

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4 nerolidylcatechol, acetic acid ethyl ester, alcohol, catechol derivative, dichloromethane, plant extract, Pothomorphe umbellata plant extract, sterol, unclassified drug, antioxidant activity, controlled study, cytotoxicity, drug solubility, drug stability, human, human cell, medicinal plant, oxidation, Piperaceae, plant leaf, Pothomorphe umbellata

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BioMed Research International, v. 2013.