Rapid detection of resistance to pyrazinamide in Mycobacterium tuberculosis using the resazurin microtitre assay
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Data
2011-05-01
Autores
Zanetti Campanerut, Paula Aline
Ghiraldi, Luciana Dias
Espinoza Spositto, Fernanda Luisa
Sato, Daisy Nakamura
Leite, Clarice Queico Fujimura [UNESP]
Hirata, Mario Hiroyuki
Crespo Hirata, Rosario Dominguez
Cardoso, Rosilene Fressatti
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Oxford University Press
Resumo
Objectives: The resazurin microtitre plate assay (REMA) was evaluated to determine the susceptibility of Mycobacterium tuberculosis to pyrazinamide, and was compared with the broth microdilution method (BMM), the absolute concentration method (ACM) and pyrazinamidase (PZase) determination.Methods: Thirty-four M. tuberculosis clinical isolates (26 susceptible and 8 resistant to pyrazinamide) and reference strains M. tuberculosis H37Rv ATCC 27294 and Mycobacterium bovis AN5 were tested.Results: REMA and BMM showed 100% specificity and sensitivity when compared with ACM; BMM, however, demanded more reading time. The PZase determination assay showed 87.50% and 100% sensitivity and specificity, respectively.Conclusions: All tested methods in this preliminary study showed excellent sensitivity and specificity for the determination of pyrazinamide susceptibility of M. tuberculosis, but REMA was faster, low-cost and easy to perform and interpret. Additional studies evaluating REMA for differentiating pyrazinamide-resistant and-susceptible M. tuberculosis should be conducted on an extended panel of clinical isolates.
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TB, mycobacteria, susceptibility testing
Como citar
Journal of Antimicrobial Chemotherapy. Oxford: Oxford Univ Press, v. 66, n. 5, p. 1044-1046, 2011.