Histologic Characterization of Engineered Tissues in the Canal Space of Closed-apex Teeth with Apical Periodontitis
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Data
2013-09-30
Autores
Gomes-Filho, João Eduardo
Tobias Duarte, Paulo Carvalho
Ervolino, Edilson [UNESP]
Bomfim, Suely Regina Mogami [UNESP]
Xavier Abimussi, Caio José
Mota da Silva Santos, Ludmilla
Lodi, Carolina Simonetti
Oliveira, Sandra Helena Penha de [UNESP]
Dezan Júnior, Eloi [UNESP]
Cintra, Luciano Tavares Angelo [UNESP]
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Introduction: The aim of this study was to investigate the capacity of endodontic regenerative procedures combining an induced blood clot, platelet-rich plasma (PRP), and bone marrow aspirate (BMA) to regenerate dental pulp in canine closed-apex necrotic teeth. Methods: Apical periodontitis was induced in 20 upper and lower premolars of 2 dogs. After biomechanical preparation, enlargement to a #60 file, and disinfection with a triantibiotic paste for 28 days, the roots were randomly assigned to 4 treatment groups: blood clot (BC), BC + PRP gel, BC + BMA gel, and BC + BMA/PRP gel. Negative controls were also included. After a 3-month follow-up period, the animals were killed. Results: Histologic analysis showed the presence of newly formed vital tissues (connective, cement-like, and bone-like tissue) in 23 of the 32 treated roots (71.87%). There was no statistically significant difference between the treatment groups. Conclusions: New vital tissues were formed and characterized as connective, cementum-like, or bone-like, but not as pulp-like tissue; PRP and/or BMA did not improve the tissue ingrowth. © 2013 American Association of Endodontists.
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Bone marrow aspirate, necrotic teeth, platelet-rich plasma, regenerative endodontics, tissue engineering, vital tissue growth
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Journal of Endodontics.