Extracellular β-fructofuranosidase from Fusarium graminearum: Stability of the spray-dried enzyme in the presence of different carbohydrates
Nenhuma Miniatura disponível
Data
2013-10-16
Autores
Gonçalves, Heloísa Bressan [UNESP]
Jorge, João Atílio
Oliveira, Wanderley Pereira
Souza, Claudia Regina Fernandes
Guimarães, Luis Henrique Souza [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
Microbial enzymes have been used for various biotechnological applications; however, enzyme stabilization remains a challenge for industries and needs to be considered. This study describes the effects of spray-drying conditions on the activity and stability of β-fructofuranosidase from Fusarium graminearum. The extracellular enzyme β-fructofuranosidase was spray dried in the presence of stabilizers, including starch (Capsul) (SC), microcrystalline cellulose (MC), trehalose (TR), lactose (LC) and β-cyclodextrin (CD). In the presence of TR (2% w/v), the enzymatic activity was fully retained. After 1 year of storage, 74% of the enzymatic activity was maintained with the CD stabilizer (10% w/v). The residual activity was maintained as high as 80% for 1 h at 70°C when MC, SC and CD (5% w/v) stabilizers were used. Spray drying with carbohydrates was effective in stabilizing the F. graminearum β-fructofuranosidase, improved enzymatic properties compared to the soluble enzyme and demonstrated a potential use in future biotechnology applications. © 2013 Informa UK Ltd. All rights reserved.
Descrição
Palavras-chave
Enzyme stabilization, Fungal enzymes, Microencapsulation, invertase, Spray drying
Como citar
Journal of Microencapsulation, v. 30, n. 7, p. 624-631, 2013.