Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping
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Data
2011-06-01
Autores
Miyata, Marcelo [UNESP]
Santos, Adolfo Carlos Barreto [UNESP]
Mendes, Natália Helena
Cunha, Eunice Atsuko
Melo, Fernando Augusto Fiúza de
Leite, Clarice Queico Fujimura
Título da Revista
ISSN da Revista
Título de Volume
Editor
Sociedade Brasileira de Microbiologia
Resumo
We report a comparative study of two DNA extraction techniques, thermolysis and chemical lysis (CTAB), for molecular identification and genotyping of M. tuberculosis. Forty DNA samples were subjected to PCR and the results demonstrated that with thermolysis it is possible to obtain useful data that enables fast identification and genotyping.
Descrição
Palavras-chave
DNA extraction, Mycobacterium tuberculosis, thermolysis, CTAB
Como citar
Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 42, n. 2, p. 774-777, 2011.