Efeito da estocagem a curto prazo e da temperatura sobre gametas de jundiá, Rhamdia quelen (Quoy & Gaimard, 1824)
Arquivos
Data
2009-08-21
Autores
Sanches, Eduardo Antônio [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Estadual Paulista (Unesp)
Resumo
O trabalho foi conduzido com o objetivo de avaliar o efeito da temperatura de estocagem a curto prazo e da temperatura da água sobre os ovócitos e sêmen de jundiá, Rhamdia quelen. O experimento foi realizado em três etapas: (1) avaliar a qualidade dos ovócitos após estocagem e ativação em diferentes temperaturas; (2) padronizar a utilização de análise espermática computadorizada por meio de softwares livres; (3) avaliar os parâmetros espermáticos computadorizados após estocagem e ativação em diferentes temperaturas. Para a primeira, utilizou-se um delineamento experimental fatorial no tempo (5×3×3×3), com os tratamentos realizados em triplicata a cada 48h, na exposição de ovócitos nas temperaturas de 15; 25 e 35ºC e, ativados com água à 15; 25 e 35ºC cada, nos períodos de: 0; 45; 90; 135 e 180 minutos pós-coleta. A segunda etapa foi realizada através da captura de vídeos da movimentação espermática pelo software AMCAP, a uma taxa de 60 frames por segundo, processados no software VIRTUALDUB e analisados no software IMAGEJ por meio do aplicativo CASA. Os seguintes parâmetros foram analisados: taxa de motilidade espermática (MOT), velocidade curvilinear (VCL), velocidade média do deslocamento (VMD), velocidade em linha reta (VLR), linearidade (LIN), balanço (BAL) e freqüência de batimentos (FBAT). Os parâmetros espermáticos foram avaliados em um segundo de imagem nos tempos de 15, 25 e 35 segundos após a ativação espermática. Para a terceira etapa foi utilizado um delineamento experimental fatorial no tempo semelhante à primeira ,com 13 tempos de avaliação pós-coleta: 0; 2; 4; 6; 8; 10; 12; 16; 20; 24; 32; 40 e 48 horas. Os tratamentos foram realizados em triplicata e em protocolos sequenciais a cada 50 horas avaliando-se os parâmetros obtidos na segunda etapa. Estes foram submetidos às análises de correlação (Pearson)...
The study was conducted to evaluate the effect of temperature of storage in the short-term and the temperature of the water on the oocytes and sperm of jundiá, Rhamdia quelen. The experiment was conducted in three stages: (1) assess the quality of oocytes after storage and activation at different temperatures; (2) standardize the use of computerized sperm analysis by software free, (3) evaluate the computerized sperm parameters after storage and activation at different temperatures. For the first, was used a factorial experimental design in time (5×3×3×3), with treatments performed in triplicate every 48 hours, the exposure of oocytes at temperatures of 15, 25 and 35 C, and activated with water to 15, 25 and 35 C each, in the periods of: 0; 45; 90; 135 and 180 minutes post-collection. The second stage was performed by video capture of the movement of sperm through AMCAP software at a rate of 60 frames per second, processed and analyzed in VirtualDub software and ImageJ software using the CASA application. The following parameters were analyzed: rate of sperm motility (MOT), velocity curvilinear (VCL), velocity average path (VAP), velocity straight line (VSL), linearity (LIN), wobble (WOB), beating cross frequency (BCF). The sperm parameters were evaluated in a second image in the times of 15, 25 and 35 seconds after sperm activation. For the third step we used a factorial experimental design similar to the first in time, with 13 times of evaluation post-collection: 0; 2; 4; 6; 8; 10; 12; 16; 20; 24; 32; 40 and 48 hours. The treatments were performed in triplicate and sequencing protocols to be evaluated every 50 hours if the parameters obtained in the second stage. These were submitted to analysis of correlation (Pearson). The significant parameters (P<0.05) were summarized by the principals components analysis. For the first stage interaction (P<0.05) between time and temperature... (Complete abstract click electronic access below)
The study was conducted to evaluate the effect of temperature of storage in the short-term and the temperature of the water on the oocytes and sperm of jundiá, Rhamdia quelen. The experiment was conducted in three stages: (1) assess the quality of oocytes after storage and activation at different temperatures; (2) standardize the use of computerized sperm analysis by software free, (3) evaluate the computerized sperm parameters after storage and activation at different temperatures. For the first, was used a factorial experimental design in time (5×3×3×3), with treatments performed in triplicate every 48 hours, the exposure of oocytes at temperatures of 15, 25 and 35 C, and activated with water to 15, 25 and 35 C each, in the periods of: 0; 45; 90; 135 and 180 minutes post-collection. The second stage was performed by video capture of the movement of sperm through AMCAP software at a rate of 60 frames per second, processed and analyzed in VirtualDub software and ImageJ software using the CASA application. The following parameters were analyzed: rate of sperm motility (MOT), velocity curvilinear (VCL), velocity average path (VAP), velocity straight line (VSL), linearity (LIN), wobble (WOB), beating cross frequency (BCF). The sperm parameters were evaluated in a second image in the times of 15, 25 and 35 seconds after sperm activation. For the third step we used a factorial experimental design similar to the first in time, with 13 times of evaluation post-collection: 0; 2; 4; 6; 8; 10; 12; 16; 20; 24; 32; 40 and 48 hours. The treatments were performed in triplicate and sequencing protocols to be evaluated every 50 hours if the parameters obtained in the second stage. These were submitted to analysis of correlation (Pearson). The significant parameters (P<0.05) were summarized by the principals components analysis. For the first stage interaction (P<0.05) between time and temperature... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Fishes reproduction, Oocytes, Peixe - Reprodução, Sêmen, Ovócitos
Como citar
SANCHES, Eduardo Antônio. Efeito da estocagem a curto prazo e da temperatura sobre gametas de jundiá, Rhamdia quelen (Quoy & Gaimard, 1824). 2009. x, 88 f. Dissertação (mestrado) - Universidade Estadual Paulista, Centro de Aqüicultura, 2009.