Carbon fiber microelectrodes for adsorptive stripping analysis of trace nucleic acids
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Data
1998-01-15
Autores
Wang, J.
Cai, X. H.
Fernandes, JR
Grant, D. H.
Ozsoz, M.
Título da Revista
ISSN da Revista
Título de Volume
Editor
Elsevier B.V.
Resumo
Carbon fiber ultramicroelectrodes are shown to be suitable for adsorptive stripping potentiometric measurements of trace DNA and RNA. The origin of the carbon fiber has a profound effect upon its suitability for trace analysis of nucleic acids, with the 'Aesar' materials performing most favorably. The resulting ultramicroelectrodes offer effective adsorptive accumulation of DNA and RNA from unstirred microliter-volume solutions, and are shown to be useful in adsorptive stripping transfer experiments. The influence of the surface pretreatment and accumulation conditions is described, along with the analytical-performance characteristics. The detection limits are 6, 15 and 40 mu g/l tRNA, ssDNA and dsDNA, respectively (5 min accumulation). (C) 1998 Elsevier B.V. S.A.
Descrição
Palavras-chave
nucleic acids, DNA, RNA, carbon fiber electrode, microelectrode, chronopotentiometry, potentiometric stripping analysis
Como citar
Journal of Electroanalytical Chemistry. Lausanne: Elsevier B.V. Sa, v. 441, n. 1-2, p. 167-172, 1998.