Cariótipo de seis espécies de Bokermannohyla dos grupos de B. circumdata e B. pseudopseudis (Anura, Hylidae)
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2008-02-26
Autores
Catroli, Glaucilene Ferreira [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
A família Hylidae passou nos últimos anos por extensas modificações na taxonomia e sistemática, realizadas com base principalmente em dados moleculares e com pouca ou nenhuma contribuição de informações citogenéticas. Como conseqüência, alguns novos gêneros de Hylidae foram criados, sendo esse o caso de Bokermannohyla, atualmente o terceiro mais abundante dentro de Cophomantini, uma das quatro tribos da subfamília Hylinae. Levando-se em conta que os dados citogenéticos no gênero são muito escassos, limitados a apenas três das 27 espécies, análises cariotípicas foram realizadas em B. circumdata, B. hylax e Bokermannohyla sp., do grupo de B. circumdata, e em B. alvarengai, B. ibitiguara e B. saxicola, do grupo de B. pseudopseudis. As seis espécies compartilham um cariótipo similar com 2n=24 e NF=48. Nas espécies do grupo de B. circumdata, as Ag-RON estão localizadas nos braços longos dos cromossomos do par 11, enquanto nas espécies do grupo de B. pseudopseudis, esse marcador citológico está nos braços curtos dos cromossomos do par 4 de B. alvarengai e nos braços longos dos cromossomos dos pares 1 e 11 de B. ibitiguara e B. saxicola, respectivamente. O padrão de bandamento C obtido em todas as espécies, com exceção de B. hylax, é predominantemente centromérico, mas algumas delas mostraram bandas pericentromérica, intersticial ou terminal, assim como banda C coincidente com o sítio da Ag-RON. Os cromossomos de Bokermannohyla sp., B. ibitiguara e B. saxicola não mostraram nenhuma região repetitiva rica em bases AT com a coloração pelo DAPI, mas a banda C telomérica dos cromossomos do par 10 de B. circumdata eram brilhantes. Com o fluorocromo GC-específico CMA3, a região centromérica dos cromossomos das quatro espécies aparece fluorescente, assim como os sítios da RON. Padrões de bandas de replicação foram obtidos em B. alvarengai e B. circumdata...
In the last years, the family Hylidae went through extensive taxonomic and systematic revisions, based mostly on molecular data with few or no contribution of cytogenetic information. As a result, some new hylid genera were erected, and this is the case of Bokermannohyla, now the third most speciose genus within Cophomantini, one of the four tribes of the subfamily Hylinae. Taking into account that cytogenetic data on the genus are very scanty, restricted to only three of its 27 species, karyotypic analyses were carried out in B. circumdata, B. hylax and Bokermannohyla sp., assigned to the B. circumdata group, and in B. alvarengai, B. ibitiguara and B. saxicola, assigned to the B. pseudopseudis group. The six species share a similar karyotype with 2n=24 and FN=48. In the species of the B. circumdata group, Ag-NOR are located in the long arms of the chromosome pair 11, whereas in species of the B. pseudopseudis group, this cytological marker is in the long arms of the chromosome pairs 1 and 11 of B. ibitiguara and B. saxicola, respectively, and in the short arms of the chromosome pair 4 of B. alvarengai. The C banding pattern obtained in all species, except for B. hylax, is mostly centromeric, but some of them showed pericentromeric, interstitial or terminal bands, as well as C band coincident to the Ag-NOR site. The chromosomes of Bokermannohyla sp., B. ibitiguara and B. saxicola showed no special AT-rich repetitive regions with DAPI staining, but the telomeric C band of the chromosome pair 10 of B. circumdata exhibited brilliant fluorescence. With the GC-specific CMA3 fluorochrome, the centromeric region of the chromosomes of the four species appeared brightly stained, as well as the NOR sites. Replication banding patterns were obtained in B. alvarengai and B. circumdata, but in the later species the chromosomes were poorly differentiated, probably due to incomplete BrdU incorporation... (Complete abstract click electronic access below)
In the last years, the family Hylidae went through extensive taxonomic and systematic revisions, based mostly on molecular data with few or no contribution of cytogenetic information. As a result, some new hylid genera were erected, and this is the case of Bokermannohyla, now the third most speciose genus within Cophomantini, one of the four tribes of the subfamily Hylinae. Taking into account that cytogenetic data on the genus are very scanty, restricted to only three of its 27 species, karyotypic analyses were carried out in B. circumdata, B. hylax and Bokermannohyla sp., assigned to the B. circumdata group, and in B. alvarengai, B. ibitiguara and B. saxicola, assigned to the B. pseudopseudis group. The six species share a similar karyotype with 2n=24 and FN=48. In the species of the B. circumdata group, Ag-NOR are located in the long arms of the chromosome pair 11, whereas in species of the B. pseudopseudis group, this cytological marker is in the long arms of the chromosome pairs 1 and 11 of B. ibitiguara and B. saxicola, respectively, and in the short arms of the chromosome pair 4 of B. alvarengai. The C banding pattern obtained in all species, except for B. hylax, is mostly centromeric, but some of them showed pericentromeric, interstitial or terminal bands, as well as C band coincident to the Ag-NOR site. The chromosomes of Bokermannohyla sp., B. ibitiguara and B. saxicola showed no special AT-rich repetitive regions with DAPI staining, but the telomeric C band of the chromosome pair 10 of B. circumdata exhibited brilliant fluorescence. With the GC-specific CMA3 fluorochrome, the centromeric region of the chromosomes of the four species appeared brightly stained, as well as the NOR sites. Replication banding patterns were obtained in B. alvarengai and B. circumdata, but in the later species the chromosomes were poorly differentiated, probably due to incomplete BrdU incorporation... (Complete abstract click electronic access below)
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Palavras-chave
Anuro, Citogenetica, Anfíbio, Cromossomos, Citotaxonomia, Evolução cromossômica, Amphibia, Cytogenetics, Anura
Como citar
CATROLI, Glaucilene Ferreira. Cariótipo de seis espécies de Bokermannohyla dos grupos de B. circumdata e B. pseudopseudis (Anura, Hylidae). 2008. 96 f. Dissertação (mestrado) - Universidade Estadual Paulista, Instituto de Biociências de Rio Claro, 2008.