Eletroforetograma de proteínas séricas de cães linfomatosos, submetidos ao protocolo quimioterápico de Madison-Wisconsina
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Data
2009-06-29
Autores
Vieira, Manuela Cristina [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
O linfoma é o principal tumor hematopoiético no cão e é caracterizado pela proliferação de células originadas do tecido linfóide, histiócitos e seus precursores. Os animais com linfoma frequentemente apresentam alterações hematológicas e bioquímico-séricas, tais como anemia normocítica normocrômica não regenerativa, anemia hemolítica, hipercalcemia e gamopatia monoclonal. O objetivo desse estudo foi quantificar e qualificar as proteínas séricas totais e suas frações, em cães sadios e linfomatosos, sendo estes submetidos ao protocolo quimioterápico de Madison- Wisconsin. Após sinérese, centrifugação e obtenção das amostras de soro, de 10 cães sadios e 10 cães linfomatosos, as proteínas de fase aguda foram separadas por eletroforese em matriz de gel de poliacrilamida, e suas concentrações determinadas por densitometria computadorizada. Foram encontradas de 18 a 31 proteínas no fracionamento eletroforético, com pesos moleculares variando de 18 a 245 KD (kilodáltons). Os pesos moleculares obtidos foram: IgA, 170 KD; ceruloplasmina, 125 KD; transferrina, 85KD; albumina, 65 KD; 1-antitripsina, 60 KD; IgG (cadeia pesada), 50 KD; haptoglobina, 39 KD; 1-glicoproteína ácida, 37 KD; proteína nº 9, 33 KD; IgG (cadeia leve), 25 KD; e proteína nº 11, 23 KD. Os resultados mostraram que algumas proteínas de fase aguda se alteram no linfoma. A 1-antitripsina, 1-glicoproteína ácida, transferrina, IgG (cadeia pesada) e globulinas apresentaram diferenças significativas entre cães sadios e linfomatosos, no momento zero, antes da 1ª sessão de quimioterapia. Já nos cães linfomatosos, somente a concentração da proteína nº 9 (33KD) apresentou diferença significativa, durante o protocolo quimioterápico.
The lymphoma is the principal hematopoietic tumor in dogs and it is characterized by the proliferation of cells from lymphoid tissue, histiocytes and its precursors. Animals with lymphoma often showed changes in biochemical and hematological parameters of those animals such as non-regenerative normocromica normocytic anemia, hemolytic anemia, hypocalcemia and monoclonal gamopatia. The purpose of this study was quantify and qualify the serum total proteins and its fraction, in dogs healthy and with lymphoma, these under went the Madison-Wisconsin chemotherapy protocol. After centrifugation and fractioning of the serum samples, the acute phase proteins were separated by polyacrilamide gel electrophoresis, and their concentrations were determined by computer densitometry. Between eighteen and thirty proteins were separated by eletrophoresis, with molecular weights ranged from 18 to 245 KD (kilodaltons). The molecular weights of the proteins more found were: IgA, 170 KD; ceruloplasmin, 125 KD; transferrin, 85 KD; albumin, 65 KD; 1-antitripsin, 60 KD; IgG (weighty chain), 50 KD; haptoglobin, 39 KD; 1-acid glycoprotein, 37 KD; protein nº 9, 33 KD; IgG (light chain), 25 KD and protein nº 11, 23 KD. The results showed that some acute phase proteins changed in lymphoma. The 1-antitripsina, 1-acid glycoprotein, transferring, IgG (weighty chain) and globulins, showed significantly diferences between healthy and lymphoma dogs, in time zero, before 1ª chemotherapy session. In dogs with lymphoma, only protein nº 9 (33KD) showed significantly diference, during chemotherapy protocol.
The lymphoma is the principal hematopoietic tumor in dogs and it is characterized by the proliferation of cells from lymphoid tissue, histiocytes and its precursors. Animals with lymphoma often showed changes in biochemical and hematological parameters of those animals such as non-regenerative normocromica normocytic anemia, hemolytic anemia, hypocalcemia and monoclonal gamopatia. The purpose of this study was quantify and qualify the serum total proteins and its fraction, in dogs healthy and with lymphoma, these under went the Madison-Wisconsin chemotherapy protocol. After centrifugation and fractioning of the serum samples, the acute phase proteins were separated by polyacrilamide gel electrophoresis, and their concentrations were determined by computer densitometry. Between eighteen and thirty proteins were separated by eletrophoresis, with molecular weights ranged from 18 to 245 KD (kilodaltons). The molecular weights of the proteins more found were: IgA, 170 KD; ceruloplasmin, 125 KD; transferrin, 85 KD; albumin, 65 KD; 1-antitripsin, 60 KD; IgG (weighty chain), 50 KD; haptoglobin, 39 KD; 1-acid glycoprotein, 37 KD; protein nº 9, 33 KD; IgG (light chain), 25 KD and protein nº 11, 23 KD. The results showed that some acute phase proteins changed in lymphoma. The 1-antitripsina, 1-acid glycoprotein, transferring, IgG (weighty chain) and globulins, showed significantly diferences between healthy and lymphoma dogs, in time zero, before 1ª chemotherapy session. In dogs with lymphoma, only protein nº 9 (33KD) showed significantly diference, during chemotherapy protocol.
Descrição
Palavras-chave
Dog, Electrophoresis, Limphoma, Chemotherapy, Acute phase proteins, Cão, Eletroforese, Linfoma, Quimioterapia, Proteína de fase aguda
Como citar
VIEIRA, Manuela Cristina. Eletroforetograma de proteínas séricas de cães linfomatosos, submetidos ao protocolo quimioterápico de Madison-Wisconsina. 2009. xvi, 91 f. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, 2009.