Avaliação da biocompatibilidade de vários elastômeros de silicone implantados no tecido subcutâneo de ratos: estudo histológico e histomorfométrico
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Data
2005-08-22
Autores
França, Diurianne Caroline Campos [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
No presente estudo foram utilizados 60 ratos submetidos a implantes subcutâneos de quatro elastômeros de silicone, sendo três usados em prótese bucomaxilofacial, LIM 6050, MDX 4-4210 e Silastic 732 RTV industrial e um indicado para cirurgia plástica, o Silimed. O objetivo do estudo foi avaliar os materiais em relação à compatibilidade biológica tecidual, nos tempos pós-operatórios de sete, quinze e trinta dias, quando os animais foram sacrificados e as peças processadas e coradas pela hematoxilina e eosina para análise qualitativa e quantitativa dos espécimes. Pelo estudo histomorfométrico, oito áreas de 60,11 mm2 foram analisadas, contando-se as células mesenquimais, inflamatórias mononucleares, eosinófilos e as células gigantes do tipo corpo estranho. O experimento foi desenvolvido em dois capítulos, estudando-se separadamente os silicones de uso em prótese e o Silimed. No primeiro capítulo, a análise da biocompatibilidade dos implantes subcutâneos dos elastômeros de silicone LIM 6050, MDX 4-4210 e Silastic 732 RTV industrial, observou-se biocompatibilidade aceitável em todos eles, considerando-se o fato de que sua indicação protética dependeria em especial das características físicas próprias de cada material, pela mínima reação tecidual observada, além de que em sua forma sólida é fácil a adaptação aos tecidos. No segundo capítulo, o estudo se baseou na análise das reações referentes ao implante de silicone utilizado em cirurgia plástica (Silimed), constatando-se a presença de processo inflamatório ligeiramente maior no silicone gel em relação aos outros grupos, porém com níveis aceitáveis de biocompatibilidade, confirmada pela rara presença de células gigantes do tipo corpo estranho. Todos os dados foram submetidos à análise de variância e teste de Tukey, demonstrando que todos os materiais implantados iniciaram uma resposta...
In the present study 60 rats were submitted to subcutaneous implant of four elastomers of silicon, being three of use in bucomaxillofacial prosthesis, LIM 6050, MDX 4-4210 and Silastic 732 RTV industrial and a suitable one for plastic surgery, Silimed. The objective of the study was to evaluate the materials in relation to the tissue biological compatibility, in the postoperative times of seven, fifteen and thirty days, when the animals were sacrificed and the processed pieces and stained for the hematoxilin and eosin for qualitative and quantitative analysis of the specimens. For the histomorphometric study, eight areas of 60,11 mm2 were analyzed, being counted the mesenchimal cells, inflammatory cells, eosinophile and giant cells. The experiment was developed in two chapters, being studied the use silicons separately in prosthesis and Silimed. In the first chapter, the analysis of the biocompatibility of the subcutaneous implant of the elastomers of silicon LIM 6050, MDX 4-4210 and Silastic 732 RTV industrial, acceptable biocompatibility was observed in all of them, being considered the fact that your prosthetic indication would especially depend on the own physical characteristics of each material, for the low tissue reaction observed, in addition in your solid form it is easy the adaptation to the tissues. In the second chapter, the study based on the analysis of the referring reactions to the it implants of silicon used in plastic surgery (silimed), being verified the presence of inflammatory process lightly larger in relation to the other groups, however with acceptable levels of biocompatibility confirmed by the rare presence of giant cells of the type strange body. All the data were submitted to the variance analysis and test of Tukey, demonstrating that all the implanted materials began an acceptable tissue inflammatory reaction, with tissue reactions of light intensity the moderate... (Complete abstract, click electronic address below)
In the present study 60 rats were submitted to subcutaneous implant of four elastomers of silicon, being three of use in bucomaxillofacial prosthesis, LIM 6050, MDX 4-4210 and Silastic 732 RTV industrial and a suitable one for plastic surgery, Silimed. The objective of the study was to evaluate the materials in relation to the tissue biological compatibility, in the postoperative times of seven, fifteen and thirty days, when the animals were sacrificed and the processed pieces and stained for the hematoxilin and eosin for qualitative and quantitative analysis of the specimens. For the histomorphometric study, eight areas of 60,11 mm2 were analyzed, being counted the mesenchimal cells, inflammatory cells, eosinophile and giant cells. The experiment was developed in two chapters, being studied the use silicons separately in prosthesis and Silimed. In the first chapter, the analysis of the biocompatibility of the subcutaneous implant of the elastomers of silicon LIM 6050, MDX 4-4210 and Silastic 732 RTV industrial, acceptable biocompatibility was observed in all of them, being considered the fact that your prosthetic indication would especially depend on the own physical characteristics of each material, for the low tissue reaction observed, in addition in your solid form it is easy the adaptation to the tissues. In the second chapter, the study based on the analysis of the referring reactions to the it implants of silicon used in plastic surgery (silimed), being verified the presence of inflammatory process lightly larger in relation to the other groups, however with acceptable levels of biocompatibility confirmed by the rare presence of giant cells of the type strange body. All the data were submitted to the variance analysis and test of Tukey, demonstrating that all the implanted materials began an acceptable tissue inflammatory reaction, with tissue reactions of light intensity the moderate... (Complete abstract, click electronic address below)
Descrição
Palavras-chave
Elastomeros na odontologia, Protese maxilo-facial, Oncologia, Boca - Cirurgia, Elastômeros de silicone, Cirurgia bucal, Ratos Wistar, Géis de silicone, Silicone elastomers, Rats, Wistar, Maxillofacial prosthesis, Medical oncology, Silicone gels
Como citar
FRANÇA, Diurianne Caroline Campos. Avaliação da biocompatibilidade de vários elastômeros de silicone implantados no tecido subcutâneo de ratos: estudo histológico e histomorfométrico. 2005. 93 f. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Odontologia de Araçatuba, 2005.