Estudo do fenótipo osteoblástico em células-tronco mesenquimais de ratos normotensos e espontaneamente hipertensos na presença ou não de doença periodontal
Carregando...
Arquivos
Data
2013-08-01
Autores
Amaral, Caril Constante Ferreira do [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Estadual Paulista (Unesp)
Resumo
Este estudo teve como objetivo avaliar o efeito da doença periodontal (DP) induzida por ligadura nos mecanismos envolvidos na atividade osteoblástica e mineralização da matriz óssea a partir de células-tronco derivadas da medula óssea do fêmur de ratos normotensos e espontaneamente hipertensos (SHR). As linhagens (180-220 g) foram divididas em dois grupos cada: Wistar Controle [WC] e SHR Controle [SC], Wistar com DP [WDP] e SHR com DP [SDP]. Foi realizada na área de furca a análise imunoistoquímica da expressão de receptor ativador nuclear kappa-B ligante (RANKL), osteoprotegerina (OPG), fosfatase alcalina específica do osso (BALP), osteocalcina (OC), metaloproteinases de matriz (MMP)-2, e MMP-9, e fosfatase ácida tartarato resistente (TRAP) da região de furca 15 dias após a indução da DP. Célulastronco mesenquimais derivadas da medula óssea (CTMs) foram coletadas para diferenciação osteogênica. Taxa de proliferação celular, conteúdo de proteína total e atividade específica da enzima fosfatase alcalina (FAL) foram analisadas nos dias 3, 7, 10 e 14. A mineralização da matriz foi analisada no dia 17. Os dados foram expressos como média ± erro padrão da média (EPM), analisados por one- ou two-way ANOVA, seguido pelo teste de Tukey ou Bonferroni, quando apropriado. RANKL foi fracamente expresso em ambas as linhagens, mas foi moderadamente expresso em animais SDP. OPG foi moderadamente expressa em animais sem PD, mas...
This study aimed to evaluate the effect of experimentally induced periodontitis in the mechanisms involved in osteoblastic activity and bone matrix mineralization from femoral bone marrow-derived stem cells in normotensive and hypertensive rats. Wistar and SHR rats (180–220 g) were divided into 2 groups each: Wistar control [WC] and SHR control [SC], and Wistar with PD [WPD] and SHR with PD [SPD] groups. Immunohistochemical analysis of receptor activator of nuclear factor kappa-B ligand (RANKL), osteoprotegerin (OPG), bone alkaline phosphatase (BALP), osteocalcin (OC), matrix metalloproteinase (MMP)-2, MMP-9, and tartrate-resistant acid phosphatase (TRAP) of the furcation region were assessed 15 days after PD. Bone marrow mesenchymal stem cells (MSCs) were collected and assessed for osteogenic differentiation. Proliferation rate, total protein content, and ALP activity were analyzed at 3, 7, 10, and 14 days. Mineralization of matrix was analyzed at 17 days. RANKL was weakly expressed in both strains, but was moderately expressed in SPD animals. OPG was moderately expressed in animals without PD, but strongly expressed in those with PD. The expression of BALP was higher in SPD than in WPD animals. Immunolabeling for OC was absent or weak in both strains and exhibited a tendency to increase in the presence of PD. MMP-2 expression was absent or weak in animals without PD, but it increased slightly in WPD animals. MMP-9 was moderately...
This study aimed to evaluate the effect of experimentally induced periodontitis in the mechanisms involved in osteoblastic activity and bone matrix mineralization from femoral bone marrow-derived stem cells in normotensive and hypertensive rats. Wistar and SHR rats (180–220 g) were divided into 2 groups each: Wistar control [WC] and SHR control [SC], and Wistar with PD [WPD] and SHR with PD [SPD] groups. Immunohistochemical analysis of receptor activator of nuclear factor kappa-B ligand (RANKL), osteoprotegerin (OPG), bone alkaline phosphatase (BALP), osteocalcin (OC), matrix metalloproteinase (MMP)-2, MMP-9, and tartrate-resistant acid phosphatase (TRAP) of the furcation region were assessed 15 days after PD. Bone marrow mesenchymal stem cells (MSCs) were collected and assessed for osteogenic differentiation. Proliferation rate, total protein content, and ALP activity were analyzed at 3, 7, 10, and 14 days. Mineralization of matrix was analyzed at 17 days. RANKL was weakly expressed in both strains, but was moderately expressed in SPD animals. OPG was moderately expressed in animals without PD, but strongly expressed in those with PD. The expression of BALP was higher in SPD than in WPD animals. Immunolabeling for OC was absent or weak in both strains and exhibited a tendency to increase in the presence of PD. MMP-2 expression was absent or weak in animals without PD, but it increased slightly in WPD animals. MMP-9 was moderately...
Descrição
Palavras-chave
Doenças periodontais, Hipertensão, Células-Tronco, Osteoporose, Osteoblastos, Periodontal diseases
Como citar
AMARAL, Caril Constante Ferreira do. Estudo do fenótipo osteoblástico em células-tronco mesenquimais de ratos normotensos e espontaneamente hipertensos na presença ou não de doença periodontal. 2013. 150 f. Dissertação (mestrado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Odontologia de Araçatuba, 2013.