Avaliação do retinol no escarro e no soro de tabagistas e pacientes com doença pulmonar obstrutiva crônica e sua relação com o processo inflamatório
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Data
2010-02-23
Autores
Amaral, Renata Antonialli Ferreira do [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
A doença pulmonar obstrutiva crônica (DPOC) e o tabagismo são doenças inflamatórias que interferem no metabolismo da vitamina A; entretanto, o comportamento do retinol nas vias aéreas destes pacientes ainda é desconhecido. Avaliar o retinol no escarro induzido e no soro de tabagistas e pacientes com DPOC e a associação destes valores com os marcadores da inflamação sistêmica e nas vias aéreas. Foram avaliados 50 pacientes com DPOC moderada a muito grave [idade média = 64,0 +/- 9,0 anos; VEF1(%) 49,9 +/- 16,8], 19 tabagistas [idade média = 49,0 +/- 6,4 anos; VEF1(%) 107,2 +/- 15,9] e 31 indivíduos controles [idade média = 48,2 +/- 8,1 anos; VEF1(%) 111,8 +/- 15,5]. Todos os indivíduos da pesquisa foram submetidos à avaliação clínica, espirometria pré e pósbroncodilatador, oximetria de pulso, avaliação da ingestão de vitamina A e do estado nutricional e coleta de sangue. A indução e coleta de escarro foi realizada em pacientes com DPOC e tabagistas. As concentrações de retinol e do fator de necrose tumoral alfa (TNF-a), interleucina (IL)-6 e IL-8 foram avaliadas no soro e no sobrenadante de escarro induzido. A concentração de PCR no soro também foi avaliada. O teste “t” de Student ou de Mann-Whitney foi utilizado para a comparação entre dois grupos. As associações entre as variáveis foram analisadas por meio do teste de correlação de Pearson ou Spearman. Para a comparação de mais de dois grupos foi utilizada ANOVA de uma via seguido do teste de Tukey ou Kruskal-Wallis seguido do teste Dunn. A correção para idade foi realizada através da análise de covariância (ANCOVA). Os valores médios de vitamina sérica foram significativamente menores nos pacientes com DPOC (1,80 [1,25-2,16] μmol/L) quando comparados aos tabagistas (2,40 [1,95-3,14]μmol/L) (p<0, 001); enquanto que, não foi observada diferença...
Chronic obstructive pulmonary disease (COPD) and tobacco smoking are inflammatory diseases that interfere with vitamin A metabolism; however, the retinol status in the airways of these patients is unknown. The aim of this study was to evaluate retinol concentration in the induced sputum and serum of current smokers and COPD patients and to study the association between these values and those of systemic and airways inflammatory markers. We evaluated 50 patients with moderate to very severe COPD [mean age= 64,0 +/- 9,0 y; FEV1 (%) 49,9 +/- 16,8], 19 current smokers [mean age = 49,0 +/- 6,4 y; FEV1(%) 107,2 +/- 15,9] and 31 control subjects [mean age = 48,2 +/- 8,1y; FEV1 (%) 111,8 +/- 15,5]. All subjects underwent to clinical evaluation, pre and post-bronchodilator spirometry, pulse oximetry, evaluation of vitamin A intake and of the nutritional status and blood sampling. Sputum induction was perfomed only in COPD patients and current smoker subjects. Retinol, tumor necrosis factor alpha (TNF-α), interleukin (IL)-6 and IL-8 were measured in serum and induced sputum. Serum concentration of C-reactive protein (CRP) was also measured. For comparison between two groups, we used either the Student “t” test or Mann- Whitney test. The associations among variables were analyzed by means of the Pearson correlation or using Spearman. For comparison of more than two groups we used one-way ANOVA followed by Tukey test and Kruskal-Wallis test followed by Dunn. The correction for age was performed by analysis of covariance (ANCOVA). Mean values of serum retinol were significantly lower in COPD patients (1,80 [1,25-2,16] μmol/L) when compared to current smokers (2,40 [1,95- 3,14]μmol/L) (p<0,001); however, values were not significantly different between control subjects (1,98 [1,82-2,32] μmol/L), current... (Complete abstract click electronic access below)
Chronic obstructive pulmonary disease (COPD) and tobacco smoking are inflammatory diseases that interfere with vitamin A metabolism; however, the retinol status in the airways of these patients is unknown. The aim of this study was to evaluate retinol concentration in the induced sputum and serum of current smokers and COPD patients and to study the association between these values and those of systemic and airways inflammatory markers. We evaluated 50 patients with moderate to very severe COPD [mean age= 64,0 +/- 9,0 y; FEV1 (%) 49,9 +/- 16,8], 19 current smokers [mean age = 49,0 +/- 6,4 y; FEV1(%) 107,2 +/- 15,9] and 31 control subjects [mean age = 48,2 +/- 8,1y; FEV1 (%) 111,8 +/- 15,5]. All subjects underwent to clinical evaluation, pre and post-bronchodilator spirometry, pulse oximetry, evaluation of vitamin A intake and of the nutritional status and blood sampling. Sputum induction was perfomed only in COPD patients and current smoker subjects. Retinol, tumor necrosis factor alpha (TNF-α), interleukin (IL)-6 and IL-8 were measured in serum and induced sputum. Serum concentration of C-reactive protein (CRP) was also measured. For comparison between two groups, we used either the Student “t” test or Mann- Whitney test. The associations among variables were analyzed by means of the Pearson correlation or using Spearman. For comparison of more than two groups we used one-way ANOVA followed by Tukey test and Kruskal-Wallis test followed by Dunn. The correction for age was performed by analysis of covariance (ANCOVA). Mean values of serum retinol were significantly lower in COPD patients (1,80 [1,25-2,16] μmol/L) when compared to current smokers (2,40 [1,95- 3,14]μmol/L) (p<0,001); however, values were not significantly different between control subjects (1,98 [1,82-2,32] μmol/L), current... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Pneumopatias obstrutivas, Tabagismo, Chronic obstructive pulmonary disease, Tobacco smoking, Retinol
Como citar
AMARAL, Renata Antonialli Ferreira do. Avaliação do retinol no escarro e no soro de tabagistas e pacientes com doença pulmonar obstrutiva crônica e sua relação com o processo inflamatório. 2010. 76 f. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Medicina de Botucatu, 2010.