Qualidade microbiológica de camarões resfriados e comercializados em feiras-livres do município de São Paulo/SP
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Data
2013-07-12
Autores
Barbosa, Liliana José [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
O trabalho objetivou caracterizar a qualidade microbiológica do camarão resfriado e comercializado em feiras-livres na cidade de São Paulo, por meio da contagem de microrganismos mesófilos, psicrotróficos, Staphylococcus coagulase positivo, isolamento de Salmonella spp. e quantificação de coliformes termotolerantes e E. coli. Para isto, selecionou-se 14 feiras do município onde foram realizadas três coletas em momentos distintos. Em cada coleta foram compradas porções do camarão vendido limpo e do camarão inteiro, estas porções deram origem aos três tipos de amostras analisadas: o camarão vendido limpo (CL), o músculo retirado de maneira asséptica a partir do camarão vendido inteiro (M), e a água de enxágue (AE), amostra obtida a partir da lavagem da superfície externa dos camarões inteiros, com água peptonada a 0,1%. Além disso, foi observada a aplicação de pontos específicos de boas práticas de manipulação de alimentos relacionados às condições higiênico-sanitárias das barracas e dos manipuladores. Verificaram-se populações de mesófilos maiores que 106 UFC.g-1 em 7,1% das amostras de M e 34,2% das amostras de CL. Em relação à população de psicrotróficos, 28,6% das amostras de M e 86,8% das amostras de CL estavam inadequadas para o consumo. Na análise de Staphylococcus coagulase positivo, duas amostras de M (4,8%) e cinco de CL (13,2%) atingiram populações maiores que 103 UFC.g-1. A maior parte das amostras de CL e M apresentou NMP de coliformes termotolerantes <3,0 por grama de amostra. A bactéria E. coli foi isolada em 22,9% das amostras de CL e 11,9% das amostras de M. As amostras de CL tendem a ter uma contaminação por mesófilos e psicrotróficos significativamente maior que as amostras de M. As amostras de AE, mostraram que uma unidade de camarão pode veicular populações médias de mesófilos, psicrotróficos e Staphylococcus coagulase...
The objective of this study was to characterize the microbiological quality of refrigerated shrimp commercialized in open market places in the city of São Paulo, through count of mesophilic, psychotrophic and coagulase positive Staphylococcus microorganisms, isolation of Salmonella spp., and quantification of thermotolerant coliforms and E. coli. Fourteen county open market places were selected for three different sample collections obtained in distinct ocasions. Each time, amounts of “clean” shrimp (deveined and peeled) and whole shrimp were purchased, generating the three types of samples analysed: clean shrimp (CL), the muscle asseptically removed from whole shrimp (M), and rinsing water (AE), which was obtained by washing the exterior of whole shrimps with 0,1% peptone water. Furthermore, specific standards of good practice in food manipulation related to hygienic and sanitary conditions of stalls and workers manipulating the shrimp were evaluated. Mesophilic populations higher than 106 UFC.g-1 were observed in 7,1% of M samples and 34,2% of CL samples. Regarding psychotrophic populations, 28,6% of M samples and 86,8% of CL samples were inadequate for consumption. Analysis of coagulase positive Staphylococcus microorganism demonstrated that two M samples (4,8%) and five CL samples (13,2%) had reached population levels higher than 103 UFC.g-1. Most CL and M samples showed thermotolerant coliforms MPN <3,0 per gram of sample. Bacteria E. coli was isolated in 22,9% of CL samples and 11,9% of M samples. CL samples tend to have significant higher mesophilic and psychotrophic contamination levels than M samples. AE samples demonstrated that one shrimp unit can spread mean population levels of mesophilic, psychotrophic and coagulase positive Staphylococcus microorganisms equivalent to 5,4 x 107, 1,1 x 1011 and 2,0 x 104 UFC.g-1, respectively. E. coli was isolated in 50% of AE samples ...
The objective of this study was to characterize the microbiological quality of refrigerated shrimp commercialized in open market places in the city of São Paulo, through count of mesophilic, psychotrophic and coagulase positive Staphylococcus microorganisms, isolation of Salmonella spp., and quantification of thermotolerant coliforms and E. coli. Fourteen county open market places were selected for three different sample collections obtained in distinct ocasions. Each time, amounts of “clean” shrimp (deveined and peeled) and whole shrimp were purchased, generating the three types of samples analysed: clean shrimp (CL), the muscle asseptically removed from whole shrimp (M), and rinsing water (AE), which was obtained by washing the exterior of whole shrimps with 0,1% peptone water. Furthermore, specific standards of good practice in food manipulation related to hygienic and sanitary conditions of stalls and workers manipulating the shrimp were evaluated. Mesophilic populations higher than 106 UFC.g-1 were observed in 7,1% of M samples and 34,2% of CL samples. Regarding psychotrophic populations, 28,6% of M samples and 86,8% of CL samples were inadequate for consumption. Analysis of coagulase positive Staphylococcus microorganism demonstrated that two M samples (4,8%) and five CL samples (13,2%) had reached population levels higher than 103 UFC.g-1. Most CL and M samples showed thermotolerant coliforms MPN <3,0 per gram of sample. Bacteria E. coli was isolated in 22,9% of CL samples and 11,9% of M samples. CL samples tend to have significant higher mesophilic and psychotrophic contamination levels than M samples. AE samples demonstrated that one shrimp unit can spread mean population levels of mesophilic, psychotrophic and coagulase positive Staphylococcus microorganisms equivalent to 5,4 x 107, 1,1 x 1011 and 2,0 x 104 UFC.g-1, respectively. E. coli was isolated in 50% of AE samples ...
Descrição
Palavras-chave
Camarão, Estafilococos, Salmonella, Escherichia coli, Alimentos - Qualidade, Quality control
Como citar
BARBOSA, Liliana José. Qualidade microbiológica de camarões resfriados e comercializados em feiras-livres do município de São Paulo/SP. 2013. iv, 86 p. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal, 2013.