Estresse oxidativo, produção de superóxido e a apoptose de neutrófilos de cães com insuficiência renal crônica
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Data
2011-08-23
Autores
Silva, Adriana Carolina Rodrigues Almeida [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
À semelhança do que ocorre em homens e gatos, o estresse oxidativo associado à insuficiência renal crônica (IRC) canina pode afetar a função dos neutrófilos. Em cães urêmicos com IRC ocorre aumento do estresse oxidativo e os neutrófilos tornam-se menos viáveis e funcionais. Dezoito cães adultos urêmicos com insuficiência renal crônica foram comparados com 10 cães adultos controle. Foram determinados o hemograma, a urinálise, o perfil bioquímico plasmático, a peroxidação lipídica plasmática, a capacidade antioxidante total (CAT) do plasma, a produção de superóxido, a taxa de viabilidade e a apoptose dos neutrófilos. As taxas plasmáticas de uréia (P<0,01), creatinina (p<0,01) e colesterol (p<0,001) de cães urêmicos foram significativamente superiores aos do controle. Em cães com uremia o volume globular (p<0,0001), a densidade urinária (p<0,015) e a peroxidação lipídica plasmática (p<0,001) foram significativamente menores quando comparado aos do grupo controle. Os neutrófilos de cães urêmicos apresentaram uma maior taxa de apoptose espontânea (0,018±0,016 vs 0,30± 0,23, p< 0,001) e induzida com Camptotecina (4,34±3,93 vs 26,29±18,52, p<0,01). Comparado aos do grupo controle, os valores médios da fluorescência gerada pela produção de superóxido dos neutrófilos isolados de cães urêmicos foi menor na presença (162,93±173,09 vs 49,10±41,22, p< 0,001) e na ausência de estimulo com PMA (1182,5± 1253,5 vs 212,9±294,25 p< 0,01). Durante o estresse oxidativo em cães urêmicos portadores de IRC ocorre uma aceleração da apoptose que diminui o número de neutrófilos viáveis e a produção neutrofílica de superóxido. Os mecanismos de como o estresse oxidativo da uremia acelera a apoptose e diminui o metabolismo oxidativo dos neutrófilos em cães permanecem a ser determinados
Similar to what occurs in men and cats oxidative stress associated with chronic renal failure (CRF) can affect the function of canine neutrophils. In uremic dogs with CRF is increased oxidative stress and neutrophils become less viable and functional. Eighteen dogs adult uremic patients with chronic renal failure were compared with 10 control adult dogs. We measured the blood count, urinalysis, serum biochemical profile, lipid peroxidation, plasma total antioxidant capacity (TAC) of plasma, the production of superoxide, the rate of viability and apoptosis of neutrophils. Plasma levels of urea (P <0.01), creatinine (p <0.01) and cholesterol (p <0.001) in uremic dogs were significantly above baseline. In dogs with uremia the packed cell volume (p <0.0001), urine specific gravity (p <0.015) and plasma lipid peroxidation (p <0.001) were significantly lower when compared to the control group. Neutrophils in uremic dogs showed a higher rate of spontaneous apoptosis (0.018 ± 0.016 vs. 0.30 ± 0.23, p <0.001) and induced with Camptothecin (4.34 ± 3.93 vs 26.29 ± 18.52, p <0.01). Compared to the control group the mean fluorescence generated by the superoxide production of neutrophils isolated from uremic dogs was lower in the presence (162.93 ± 49.10 vs 173.09 ± 41.22, p <0.001) and in the absence of stimulation with PMA (1182.5 ± 212.9 vs. 1253.5 ± 294.25 p <0.01). During the oxidative stress in uremic dogs with CRF is an acceleration of apoptosis which reduces the number of viable neutrophils and neutrophil production of superoxide. As the mechanisms of oxidative stress in uremia accelerates apoptosis and oxidative metabolism of neutrophils in dogs it remains to be determined
Similar to what occurs in men and cats oxidative stress associated with chronic renal failure (CRF) can affect the function of canine neutrophils. In uremic dogs with CRF is increased oxidative stress and neutrophils become less viable and functional. Eighteen dogs adult uremic patients with chronic renal failure were compared with 10 control adult dogs. We measured the blood count, urinalysis, serum biochemical profile, lipid peroxidation, plasma total antioxidant capacity (TAC) of plasma, the production of superoxide, the rate of viability and apoptosis of neutrophils. Plasma levels of urea (P <0.01), creatinine (p <0.01) and cholesterol (p <0.001) in uremic dogs were significantly above baseline. In dogs with uremia the packed cell volume (p <0.0001), urine specific gravity (p <0.015) and plasma lipid peroxidation (p <0.001) were significantly lower when compared to the control group. Neutrophils in uremic dogs showed a higher rate of spontaneous apoptosis (0.018 ± 0.016 vs. 0.30 ± 0.23, p <0.001) and induced with Camptothecin (4.34 ± 3.93 vs 26.29 ± 18.52, p <0.01). Compared to the control group the mean fluorescence generated by the superoxide production of neutrophils isolated from uremic dogs was lower in the presence (162.93 ± 49.10 vs 173.09 ± 41.22, p <0.001) and in the absence of stimulation with PMA (1182.5 ± 212.9 vs. 1253.5 ± 294.25 p <0.01). During the oxidative stress in uremic dogs with CRF is an acceleration of apoptosis which reduces the number of viable neutrophils and neutrophil production of superoxide. As the mechanisms of oxidative stress in uremia accelerates apoptosis and oxidative metabolism of neutrophils in dogs it remains to be determined
Descrição
Palavras-chave
Insuficiencia renal cronica - Cão, Imunossupressão, Peroxidação lipídica, Chronic renal insufficiency - Dog, Immune suppression
Como citar
SILVA, Adriana Carolina Rodrigues Almeida. Estresse oxidativo, produção de superóxido e a apoptose de neutrófilos de cães com insuficiência renal crônica. 2011. 57 f. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Medicina Veterinária, 2011.