Análise do gene E6 de HPV de baixo risco em papilomatose de laringe
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2013-06-26
Autores
Matos, Renata Prandini Adum de [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
A Papilomatose Respiratória Recorrente (PRR) é uma doença caracterizada pela presença de tumores benignos no trato respiratório superior, sendo a laringe o sítio de lesão mais comum. Esta doença tem uma distribuição de idade bimodal, permitindo sua classificação em papilomatose juvenil ou adulta. O principal agente etiológico da PRR é o Papilomavírus Humano (HPV), um grupo de vírus de DNA, dos quais mais de 150 tipos já foram identificados. HPV-6 e HPV-11 são os tipos mais encontrados em PRR. Há poucos estudos sobre a distribuição das variantes moleculares de HPV de baixo risco. Desta maneira, o objetivo deste estudo foi avaliar a variabilidade genética do gene E6 entre isolados de HPV-6 e HPV-11 detectados em amostras de papilomatose respiratória recorrente (PRR) obtidas em uma coorte de pacientes brasileiros. A fim de comparar as sequências de nucleotídeos identificados em nosso estudo com isolados previamente reportados provenientes de outras partes do mundo, e de diferentes sítios anatômicos (papilomatose de laringe, verrugas genitais, câncer cervical e esfregaço anal), foi realizada a análise filogenética para determinar as relações filogenéticas das variantes detectadas no Brasil com as variantes isoladas em outras regiões do mundo. A região codificante completa do gene E6 de 25 amostras foi clonada e sequenciada. Em 18 isolados foi detectado o DNA do HPV-6 (72%), e em 7 isolados o DNA do HPV-11 (28%). Um total de quatro variantes genômicas diferentes de HPV-6 e duas variantes genômicas de HPV-11 foram identificadas e nenhuma variante pode ser associada com o quadro clínico do paciente. Para a reconstrução filogenética foram utilizadas as sequências de E6 detectadas neste estudo adicionalmente às sequências anteriormente publicadas originárias da Eslovênia e da África do Sul. Devido ao pequeno...
Recurrent respiratory papillomatosis (RRP) is a disease characterized by benign neoplasms and can occur anywhere within the upper respiratory tract, but the most common lesion site is the larynx. This disease has a bimodal age distribution which forms the basis of its classification as juvenile or adult. The main etiological agent of RRP is Human Papillomavirus virus (HPV), a group of DNA virus with more than 150 identified types. HPV-6 and 11 are the most common types identified in RRP. There are few studies about the distribution of natural molecular variants of low-risk HPVs. So, the aim of this study was to evaluate the E6 early gene variability among HPV-6 and HPV-11 isolates detected in recurrent respiratory papillomatosis (RRP) samples obtained in a cohort of Brazilian patients. In order to compare nucleotide sequences identified in our study with previously reported sequences isolates from different anatomic sites (laryngeal papillomas, genital warts, cervical cancer and anal swabs) obtained from other parts of the world was performed phylogenetic analysis to determine the phylogenetic relationships of variants detected in Brazil with variants isolated in others regions of world. The complete coding region of the E6 gene of 25 samples were cloned and sequenced. HPV-6 DNA was detect in 18 isolates (72%) and HPV-11 DNA in 7 isolates (28%). A total of four different HPV-6 genomic variants and two HPV-11 genomic variants were identified and any variant could not be associated with the clinical outcome. Phylogenetic trees for both HPV types were reconstructed enclosing E6 sequences detected in our study in addition to formerly published sequences from Slovenia and South Africa. The small number of samples analyzed prevents the evaluation of the association between specific molecular variants and the... (Complete abstract click electronic access below)
Recurrent respiratory papillomatosis (RRP) is a disease characterized by benign neoplasms and can occur anywhere within the upper respiratory tract, but the most common lesion site is the larynx. This disease has a bimodal age distribution which forms the basis of its classification as juvenile or adult. The main etiological agent of RRP is Human Papillomavirus virus (HPV), a group of DNA virus with more than 150 identified types. HPV-6 and 11 are the most common types identified in RRP. There are few studies about the distribution of natural molecular variants of low-risk HPVs. So, the aim of this study was to evaluate the E6 early gene variability among HPV-6 and HPV-11 isolates detected in recurrent respiratory papillomatosis (RRP) samples obtained in a cohort of Brazilian patients. In order to compare nucleotide sequences identified in our study with previously reported sequences isolates from different anatomic sites (laryngeal papillomas, genital warts, cervical cancer and anal swabs) obtained from other parts of the world was performed phylogenetic analysis to determine the phylogenetic relationships of variants detected in Brazil with variants isolated in others regions of world. The complete coding region of the E6 gene of 25 samples were cloned and sequenced. HPV-6 DNA was detect in 18 isolates (72%) and HPV-11 DNA in 7 isolates (28%). A total of four different HPV-6 genomic variants and two HPV-11 genomic variants were identified and any variant could not be associated with the clinical outcome. Phylogenetic trees for both HPV types were reconstructed enclosing E6 sequences detected in our study in addition to formerly published sequences from Slovenia and South Africa. The small number of samples analyzed prevents the evaluation of the association between specific molecular variants and the... (Complete abstract click electronic access below)
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Palavras-chave
Microbiologia médica, Virologia medica, Doenças do virus do papiloma, Virus do papiloma, Laringe - Filogenia, Medical microbiology
Como citar
MATOS, Renata Prandini Adum de. Análise do gene E6 de HPV de baixo risco em papilomatose de laringe. 2013. 72 f. Dissertação (mestrado) - Universidade Estadual Paulista Julio de Mesquita Filho. Instituto de Biociências, Letras e Ciências Exatas de São José do Rio Preto, 2013.