Ação antifúngica do resíduo líquido integral e fracionado do desfibramento da folha do sisal (Agave sisalana) sobre Malassezia pachydermatis, Tricophyton mentagrophytes e Candida albicans
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Data
2013-05-09
Autores
Almeida, Eric César de Sousa Lima [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
O tratamento das infecções micóticas, incluindo as dermatofitoses, tem sido motivo de muita preocupação em todo o mundo. Esse fato é justificado pelo aumento da prevalência dessas doenças no cenário mundial, pela necessidade de ampliação dos poucos antifúngicos hoje disponíveis, e pelo aparecimento de cepas resistentes aos antifúngicos empregados na terapêutica clínica. No presente estudo visou-se testar a atividade antifúngica do precipitado, parte sólida proveniente da centrifugação do suco extraído da mucilagem, resíduo obtido do desfibramento da folha do sisal (Agave sisalana). A mucilagem foi processada por prensagem ou trituração e o suco obtido foi submetido a diferentes técnicas de purificação através de extrações por solventes orgânicos. O precipitado foi avaliado quanto à atividade antimicrobiana tanto em sua forma úmida como seca; que após secagem, este foi reidratado em água para a obtenção de um concentrado. Com esses tipos de tratamentos, foram realizados testes de Concentração Inibitória Mínima (CIM) pela técnica de macrodiluição em tubos, a fim de determinar ação antifúngica contra os micro-organismos descritos. Para o precipitado obtido da centrifugação do suco proveniente da prensagem da mucilagem, a CIM foi de: 98 μg/mL para Candida albicans, 783 μg/mL para Tricophyton mentagrophytes e 3125 μg/mL para Malassezia pachydermatis; resultados expressos em base seca. Para o precipitado obtido da centrifugação do suco resultante da trituração e filtração da mucilagem, a CIM foi de 128 μg/mL para C. albicans, 512 μg/mL para T. mentagrophytes e 2965 μg/mL M. pachydermatis, resultados em base seca. Na segunda etapa os precipitados secos após fracionamento com os solventes orgânicos foram reidratados em água e avaliados para os testes de CIM. A fração hexânica do precipitado do suco...
The treatment of fungal infections, including dermatophytosis, has been the subject of intense concern worldwide. This fact is explained by the increased prevalence of these diseases on the global stage, the need for expansion of the few antifungal drugs available today, and the emergence of strains resistant to antifungal agents used in clinical therapy. The present study aimed to test the antifungal activity of the precipitate, the solid part from the centrifugation of the juice extracted mucilage residue obtained from shredding the leaf of sisal (Agave sisalana). The mucilage was processed by pressing or crushing and the juice obtained was subjected to various purification techniques using extractions by organic solvents. The wet and dried precipitate were evaluated for antimicrobial activity both in the wet and dry, which, after drying, this was rehydrated in water to obtain a concentrate. After these treatments, tests of Minimum Inhibitory Concentration (MIC) were performed by the macrodilution technique in tubes, in order to determine antifungal action against the microorganisms described. The precipitate obtained by centrifuged juice from the pressing of mucilage, showed MIC = 98 μg/mL for Candida albicans, 783 μg/mL for Trichophyton mentagrophytes and 3125 μg/mL for Malassezia pachydermatis, results expressed on dried basis. The precipitate obtained by centrifuged juice from the crushing and filtration of mucilage, the MIC was 128 μg/mL for C albicans, 512 μg/mL for T. mentagrophytes and 2965 μg/mL for M. pachydermatis results on dried basis. In a second step, the new precipitates obtained from the fractionation of the dried precipitate with organic solvents were rehydrated in water and evaluated for MIC testing. The hexane fraction of the precipitate juice obtained from the pressing of mucilage had a remarkable... (Complete abstract click electronic access below)
The treatment of fungal infections, including dermatophytosis, has been the subject of intense concern worldwide. This fact is explained by the increased prevalence of these diseases on the global stage, the need for expansion of the few antifungal drugs available today, and the emergence of strains resistant to antifungal agents used in clinical therapy. The present study aimed to test the antifungal activity of the precipitate, the solid part from the centrifugation of the juice extracted mucilage residue obtained from shredding the leaf of sisal (Agave sisalana). The mucilage was processed by pressing or crushing and the juice obtained was subjected to various purification techniques using extractions by organic solvents. The wet and dried precipitate were evaluated for antimicrobial activity both in the wet and dry, which, after drying, this was rehydrated in water to obtain a concentrate. After these treatments, tests of Minimum Inhibitory Concentration (MIC) were performed by the macrodilution technique in tubes, in order to determine antifungal action against the microorganisms described. The precipitate obtained by centrifuged juice from the pressing of mucilage, showed MIC = 98 μg/mL for Candida albicans, 783 μg/mL for Trichophyton mentagrophytes and 3125 μg/mL for Malassezia pachydermatis, results expressed on dried basis. The precipitate obtained by centrifuged juice from the crushing and filtration of mucilage, the MIC was 128 μg/mL for C albicans, 512 μg/mL for T. mentagrophytes and 2965 μg/mL for M. pachydermatis results on dried basis. In a second step, the new precipitates obtained from the fractionation of the dried precipitate with organic solvents were rehydrated in water and evaluated for MIC testing. The hexane fraction of the precipitate juice obtained from the pressing of mucilage had a remarkable... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Fungi, Fungos, Sisal, Mucilagem, Produtos de ação antimicrobiana, Antimicóticos
Como citar
ALMEIDA, Eric César de Sousa Lima. Ação antifúngica do resíduo líquido integral e fracionado do desfibramento da folha do sisal (Agave sisalana) sobre Malassezia pachydermatis, Tricophyton mentagrophytes e Candida albicans. 2013. 94 f. Dissertação - (mestrado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Instituto de Biociências de Rio Claro, 2013.