Efeitos in vitro de extratos naturais sobre Enterococcus faecalis, Staphylococcus aureus, Escherichia coli e endotoxinas em canais radiculares
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2010-05-03
Autores
Brito, Emanuele Gonçalves [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
A proposta deste trabalho foi avaliar a efetividade de extratos naturais, como soluções irrigadoras, sobre E. faecalis, S. aureus, E. coli, e endotoxinas (LPS) em canais radiculares. Foi avaliado também o efeito citotóxico do conteúdo do canal, após os tratamentos, pela produção de TNF-α e IL-1β em cultura de macrófagos. Foram utilizados 84 dentes unirradiculados, contaminados com associação dos três microrganismos por 28 dias. Após, foram divididos em 7 grupos (n=12), de acordo com o irrigante utilizado: G1) solução fisiológica (controle); G2) solução de clorexidina 2%; G3) extrato glicólico de própolis 12%; G4) extrato glicólico de alcaçuz 20%; G5) extrato glicólico de barbatimão 20%; G6) extrato glicólico de romã 20%; G7) extrato glicólico de cavalinha 20%. Foram realizadas 2 coletas do conteúdo do canal radicular: imediatamente após a instrumentação e após 7 dias da instrumentação. Para todas as coletas, foi realizada avaliação da atividade antimicrobiana pela contagem de UFC/mL em meios de cultura seletivos, verificação da neutralização de endotoxinas pelo teste cinético cromogênico do lisado de amebócitos de Limulus (LAL) e avaliação dos efeitos citotóxicos do conteúdo do canal pela produção de IL-1 e TNF-α por macrófagos. Os resultados foram analisados estatisticamente (ANOVA e teste de Tukey, 5%). Todos os extratos promoveram significativa redução microbiana em relação ao controle na 1ª coleta após instrumentação (p<0,05). Com relação E. faecalis, os extratos de própolis (G3), barbatimão (G5), romã (G6) e cavalinha (G7) promoveram ausência de crescimento microbiano em muitos espécimes, sendo semelhantes a clorexidina e diferentes dos demais grupos (p<0,05). Com relação S. aureus, os extratos de própolis (G3) e barbatimão (G5) promoveram ausência de crescimento microbiano em todos os espécimes, sendo semelhantes...
The purpose of this study was to evaluate the effects of natural extracts as irrigating solutions on E. faecalis, S. aureus, E. coli, and endotoxins (LPS) in root canals. The post treatment citotoxic effect of root canal content was also evaluated by TNF-α and IL-1β production by macrophages cell culture. A total of 84 single root canal teeth were contaminated with E. faecalis, S. aureus and E. coli for 28 days. The groups were assigned into 7 groups (n=12) according to the irrigant solution used: G1) physiologic solution (control); G2) chlorhexidine 2%; G3) propolis glycolic extract 12%; G4) liquorice glycolic extract 20%; G5) barbatiman glycolic extract 20%; G6) pomegranate glycolic extract 20%; G7) horsetail glycolic extract 20%. Samples were twice collected from root canals: immediately after instrumentation and 7 days after instrumentation. All samples were submitted to antimicrobial evaluation by CFU/mL counting on selective culture media. The endotoxin detoxification was evaluated by Limulus amebocyte lysate assay (LAL), and the citotoxic effects was evaluated by TNF-α and IL-1β production by macrophages cell culture. The results were statistically analysed (ANOVA and Tukey, 5%). All the extracts promoted significant microbial reduction compared to the control group on samples collected immediately after instrumentation (p<0,05). E. faecalis results showed that propolis (G3), barbatiman (G5), pomegranate (G6) and horsetail (G7) extracts promoted no microbial growth in all samples, similar to chlorhexidine solution and different from other groups (p<0,05). S. aureus results showed that propolis (G3) and barbatiman (G5) promoted absence of microbial growth in all samples, similar to chlorhexidine solution and different from other groups (p<0,05). E. coli results showed that propolis (G3), pomegranate (G6) and horsetail (G7) promoted... (Complete abstract click electronic access below)
The purpose of this study was to evaluate the effects of natural extracts as irrigating solutions on E. faecalis, S. aureus, E. coli, and endotoxins (LPS) in root canals. The post treatment citotoxic effect of root canal content was also evaluated by TNF-α and IL-1β production by macrophages cell culture. A total of 84 single root canal teeth were contaminated with E. faecalis, S. aureus and E. coli for 28 days. The groups were assigned into 7 groups (n=12) according to the irrigant solution used: G1) physiologic solution (control); G2) chlorhexidine 2%; G3) propolis glycolic extract 12%; G4) liquorice glycolic extract 20%; G5) barbatiman glycolic extract 20%; G6) pomegranate glycolic extract 20%; G7) horsetail glycolic extract 20%. Samples were twice collected from root canals: immediately after instrumentation and 7 days after instrumentation. All samples were submitted to antimicrobial evaluation by CFU/mL counting on selective culture media. The endotoxin detoxification was evaluated by Limulus amebocyte lysate assay (LAL), and the citotoxic effects was evaluated by TNF-α and IL-1β production by macrophages cell culture. The results were statistically analysed (ANOVA and Tukey, 5%). All the extracts promoted significant microbial reduction compared to the control group on samples collected immediately after instrumentation (p<0,05). E. faecalis results showed that propolis (G3), barbatiman (G5), pomegranate (G6) and horsetail (G7) extracts promoted no microbial growth in all samples, similar to chlorhexidine solution and different from other groups (p<0,05). S. aureus results showed that propolis (G3) and barbatiman (G5) promoted absence of microbial growth in all samples, similar to chlorhexidine solution and different from other groups (p<0,05). E. coli results showed that propolis (G3), pomegranate (G6) and horsetail (G7) promoted... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Canal radicular - Irrigantes, Extratos naturais, Citocinas, Endotoxinas, Natural extracts, Endotoxins, Citokines
Como citar
BRITO, Emanuele Gonçalves. Efeitos in vitro de extratos naturais sobre Enterococcus faecalis, Staphylococcus aureus, Escherichia coli e endotoxinas em canais radiculares. 2010. 130 f. : il. Dissertação (mestrado) – Universidade Estadual Paulista, Faculdade de Odontologia de São José dos Campos, 2010.