Efeito da movimentação ortodôntica na progressão da doença periodontal induzida em ratos
Carregando...
Data
2010-03-29
Autores
Nogueira, Andressa Vilas Boas [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Estadual Paulista (Unesp)
Resumo
O movimento ortodôntico ocorre por meio da remodelação dos tecidos periodontais em resposta à aplicação de uma força mecânica, sendo que vários mediadores químicos são sintetizados e liberados para seu início e manutenção. Dentre esses mediadores, o óxido nítrico (NO) atua como modulador da atividade de osteoclastos e osteoblastos na remodelação óssea. Interleucina-1_ (IL-1-_), interleucina-6 (IL-6) e fator de necrose tumoral-_ (TNF-_) estimulam a reabsorção óssea e encontram-se aumentados no movimento ortodôntico. Estes mesmos mediadores também encontram-se aumentados e participam da destruição tecidual na doença periodontal. O objetivo deste estudo é avaliar in vivo a expressão de IL-1b, IL-6, TNF-_, NO sintase constitutiva endotelial (ecNOS), e NO sintase indutível (iNOS) nos tecidos periodontais de dentes sob movimentação ortodôntica em condições de saúde e doença periodontal, correlacionando-as às alterações histomorfométricas nesses tecidos. Para isso, 88 ratos foram divididos nos grupos: 1-controle (CONTR), 2- doença periodontal experimental induzida por ligaduras (DP), 3- movimento ortodôntico (MO), 4- doença periodontal experimental induzida por ligaduras, seguido de movimento ortodôntico (MODP). Biópsias dos tecidos gengivais ao redor 13 dos primeiros molares foram removidas para análise da expressão protéica e de mRNA, respectivamente, por Western blot e RT-PCR em tempo real. Cortes histológicos foram obtidos para avaliação histomorfométrica da perda óssea alveolar. Análise estatística foi realizada através do one-way ANOVA e a comparação entre os grupos foi feita com o teste de Tukey (nível de significância de 5%). Resultado: Máxima expressão gênica de todas as proteínas avaliadas foi observada no grupo MODP, nos períodos de 3h (eNos) ou de 1 dia (citocinas e iNOS) (p<0.05)...
Orthodontic tooth movement (OTM) occurs in response to mechanical forces through periodontal tissues remodeling. Several chemical mediators are synthesized and released for the beginning and maintenance of tissue remodeling. Among these mediators, nitric oxide (NO) acts as modulator of osteoclasts and osteoblasts activities in bone turnover. Interleukin-1_ (IL-1_), interleukin-6 (IL-6) and tumor necrosis factor-_ (TNF-_) stimulate bone resorption and are increased in orthodontic movement. These same signaling molecules are also increased and play an important role on tissue destruction in periodontal disease. Objectives: The aim of this study was to assess in vivo the expression of IL-1ß, IL-6, TNF-_, NO synthase constitutive endothelial (ecNOS), and inducible NO synthase (iNOS) in dental tissues during orthodontic tooth movement in rats suffering from ligature-induced periodontal disease or in periodontally health animals. Cytokines expressions were correlated to histomorphometric changes on alveolar bone and other periodontal tissues. Methods: Eighty-eight rats were divided into four groups: 1- negative control (sham operated), 2 - ligature-induced periodontal disease (PD), 3 - orthodontic movement (OM), 4 - ligature-induced periodontal disease, followed by orthodontic movement (OMPD). Biopsies of gingival tissue around the first molars were removed for protein and mRNA expression analysis, respectively by, Western blot and Real Time RT-PCR. Histologic tissue sections were obtained for histomorphometric evaluation of alveolar bone loss. Statistical analysis was performed by one-way ANOVA and Tukey´s posthoc tests for all parameters (5% significance level). Results: Maximum mRNA expression of all evaluated proteins was observed in the OMPD group, on 3h (eNos) or day 1 (citokines and iNos) periods (p<0.05). Also on day 1, all proteins were expressed in high levels for all groups... (Complete abstract click electronic access below)
Orthodontic tooth movement (OTM) occurs in response to mechanical forces through periodontal tissues remodeling. Several chemical mediators are synthesized and released for the beginning and maintenance of tissue remodeling. Among these mediators, nitric oxide (NO) acts as modulator of osteoclasts and osteoblasts activities in bone turnover. Interleukin-1_ (IL-1_), interleukin-6 (IL-6) and tumor necrosis factor-_ (TNF-_) stimulate bone resorption and are increased in orthodontic movement. These same signaling molecules are also increased and play an important role on tissue destruction in periodontal disease. Objectives: The aim of this study was to assess in vivo the expression of IL-1ß, IL-6, TNF-_, NO synthase constitutive endothelial (ecNOS), and inducible NO synthase (iNOS) in dental tissues during orthodontic tooth movement in rats suffering from ligature-induced periodontal disease or in periodontally health animals. Cytokines expressions were correlated to histomorphometric changes on alveolar bone and other periodontal tissues. Methods: Eighty-eight rats were divided into four groups: 1- negative control (sham operated), 2 - ligature-induced periodontal disease (PD), 3 - orthodontic movement (OM), 4 - ligature-induced periodontal disease, followed by orthodontic movement (OMPD). Biopsies of gingival tissue around the first molars were removed for protein and mRNA expression analysis, respectively by, Western blot and Real Time RT-PCR. Histologic tissue sections were obtained for histomorphometric evaluation of alveolar bone loss. Statistical analysis was performed by one-way ANOVA and Tukey´s posthoc tests for all parameters (5% significance level). Results: Maximum mRNA expression of all evaluated proteins was observed in the OMPD group, on 3h (eNos) or day 1 (citokines and iNos) periods (p<0.05). Also on day 1, all proteins were expressed in high levels for all groups... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Doenças periodontais, Oxido nitrico, Citocinas, Movimentação dentária, Tooth movement, Periodontal diseases, Nitric oxide, Cytokines
Como citar
NOGUEIRA, Andressa Vilas Boas. Efeito da movimentação ortodôntica na progressão da doença periodontal induzida em ratos. 2010. 94 f. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Odontologia de Araraquara, 2010.