Celularidade endotelial na ceraoplastia penetrante em coelhos
Data
2013-05-28
Autores
Marinho, Fábio Andrade [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
Afecções que afligem o estroma corneal profundo, como as distrofias, o ceratocone, os abscessos e os sequestros da córnea são de relativa frequência. Visando ao seu tratamento, tais desordens podem ser manejadas empregando-se os transplantes corneais penetrantes. Buscou-se, com a pesquisa, avaliarem-se as condições da celularidade endotelial, à microscopia especular de não contato, à histologia e à microscopia eletrônica de varredura, na ceratoplastia penetrante em coelhos. Foram utilizados 18 animais, sadios, adultos, machos e fêmeas, com peso médio de 3,0 kg, da raça Nova Zelândia Branco. Seis grupos foram constituídos, contendo 3 animais cada. A microscopia especular foi empregada. As avaliações foram realizadas diariamente, até se completarem 30 dias e a cada três dias, até se completarem 60 dias das ceratoplastias. Colhidas, as córneas foram avaliadas à histologia e à microscopia eletrônica de varredura. Empregou-se análise de variância para medidas repetidas, com posterior avaliação pelo teste Tukey, considerando-se o nível mínimo de significância p ≤ 0,05. No pós-operatório, em face das alterações na superfície ocular, a realização da microscopia especular de não contato foi prejudicada. À histologia, evidenciaram-se edema acentuado e discreta organização estromal nas córneas receptora e doadora, ao início das avaliações. À microscopia eletrônica de varredura, não se evidenciou migração de células endoteliais do leito receptor para o doador
Diseases affecting the deep corneal stroma such as dystrophies, keratocone, abscesses and corneal sequestrum occur frequently. Those alterations may be managed by penetrating corneal transplants. On the present research the endothelial cellularity of rabbits submitted to penetrating keratoplasty was evaluated by non-contact specular microscopy, histology and scanning electron microscopy. Eighteen adult, healthy, males and females rabbits, weighting around 3.0 kg, breed New Zealand White were used. Animals were randomly assigned on 6 groups composed of 3 individuals each. Specular microscopy was employed. The animals were daily evaluated for 30 days, and every third day, until 60 days following the keratectomies. Following harvesting, the corneas were submitted to histology and scanning electron microscopy. Repeated measures Anova and Tukey's test were used for statistical analysis. Results were considered significant if p ≤ 0.05. Non-contact specular microscopy was not possible if alterations of the ocular surface were present during the post-operative period. Histological evaluation exhibited severe edema and mild stromal organization of the recipient and donor cornea during the initial post-operative period. The scanning electron microscopy, there was no endothelial cells migration from the recipient to the donor corneas
Diseases affecting the deep corneal stroma such as dystrophies, keratocone, abscesses and corneal sequestrum occur frequently. Those alterations may be managed by penetrating corneal transplants. On the present research the endothelial cellularity of rabbits submitted to penetrating keratoplasty was evaluated by non-contact specular microscopy, histology and scanning electron microscopy. Eighteen adult, healthy, males and females rabbits, weighting around 3.0 kg, breed New Zealand White were used. Animals were randomly assigned on 6 groups composed of 3 individuals each. Specular microscopy was employed. The animals were daily evaluated for 30 days, and every third day, until 60 days following the keratectomies. Following harvesting, the corneas were submitted to histology and scanning electron microscopy. Repeated measures Anova and Tukey's test were used for statistical analysis. Results were considered significant if p ≤ 0.05. Non-contact specular microscopy was not possible if alterations of the ocular surface were present during the post-operative period. Histological evaluation exhibited severe edema and mild stromal organization of the recipient and donor cornea during the initial post-operative period. The scanning electron microscopy, there was no endothelial cells migration from the recipient to the donor corneas
Descrição
Palavras-chave
Coelho, Olhos - Cirurgia, Córnea, Endotelio, Oftalmologia veterinaria, Veterinary ophthalmology
Como citar
MARINHO, Fábio Andrade. Celularidade endotelial na ceraoplastia penetrante em coelhos. 2013. vii, 33 p. Dissertação (mestrado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal, 2013.