Imunomarcação da óxido nítrico sintase induzível e da metaloproteinase-9 em úlceras corneais químicas de coelhos (Oryctolagus cuniculus - Linnaeus, 1758), tratadas com cetorolaco de trometamina
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Data
2013-02-28
Autores
Lima, Tiago Barbalho [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
Estudaram-se os efeitos do cetorolaco de trometamina à 0,5%, sem conservante, quanto ao tempo de epitelização, a alterações clínicas, histológicas (HE), sobre expressão da Óxido Nítrico Sintase induzível (iNOS) e da Metaloproteinase-9 (MMP-9) na ceratite ulcerativa causada por álcali em coelho. Doze olhos foram tratados (GT) com o fármaco e outros 12 com solução salina (GC), imediatamente à ocorrência das úlceras e a cada 6 horas. Decorridas 24h, seis córneas (n=6) de cada grupo foram colhidas (primeiro momento). As demais (n=6), o foram após a reepitelização (segundo momento). Em ambos os momentos, avaliaram-se o infiltrado inflamatório e as condições do epitélio neoformado (HE). Por imunoistoquímica, a imunomarcação de iNOS e de MMP-9 foi avaliada. A média do tempo de epitelização no GT foi de 55 horas. No GC, ele foi de 44 horas (p<0,05). Às 24 h, as córneas do GT apresentaram menor exsudação inflamatória (p<0,01), comparativamente ao GC. No segundo momento, o GT mostrou discreta exsudação inflamatória (p>0,05) e camada epitelial simples. O GC mostrou maior estratificação no epitélio neoformado. A média de imunomarcação de iNOS em células do estroma foi maior em GT, no primeiro e segundo momentos, comparativamente a GC (p>0.05). No segundo momento, a região central expressou mais iNOS, comparativamente à periférica, tanto em GT (p<0,01), quanto em GC (p<0,05). No epitélio, não se observaram diferenças significativas dos escores de imunomarcação de iNOS entre os grupos e os momentos (p=0.69). Os escores de imunomarcação para MMP-9, no epitélio, foram menores em GT, comparativamente a GC (p=0,69). A média de imunomarcação de MMP-9 em células do estroma, no segundo momento, foi maior na região central, comparativamente à periférica (p=0.32). Não houve correlação entre a...
We studied the effects of ketorolac tromethamine at 0.5% without preservative, about the time of epithelialization, the clinical, histological (HE) on the expression of inducible Nitric Oxide Synthase (iNOS) and metalloproteinase-9 (MMP -9) in ulcerative keratitis in rabbits caused by alkali. Twelve eyes were treated (GT) to the drug and 12 with saline (GC), immediately to the occurrence of ulcers and every 6 hours. After 24h, six corneas (n = 6) of each group were harvested (first moment). The other (n = 6), were after reepithelialization (second time). In both instances, we evaluated the inflammatory infiltrate and conditions of the newly formed epithelium (HE). For immunohistochemistry, immunostaining of iNOS and MMP-9 was assessed. The average time of epithelialization in GT was 55 hours. In the GC, it was 44 hours (p <0.05). At 24 h, the corneas of GT showed lower inflammatory exudation (p <0.01) compared to the CG. In the second phase, the GT revealed mild inflammatory exudation (p> 0.05) and simple epithelial layer. The GC showed greater stratification in the newly formed epithelium. The average immunostaining of iNOS in stromal cells was higher in GT, the first and second moments compared to control group (p> 0.05). In the second step, the central region expressed more iNOS compared to peripheral in both TG (p <0.01) and in control group (p <0.05). In the epithelium, there were no significant differences in scores of iNOS immunostaining between groups and time (p = 0.69). The scores of immunostaining for MMP-9 in the epithelium were lower in GT compared to GC (p = 0.69). The average immunostaining of MMP-9 in stromal cells, the second time was greater in the central region as compared to the peripheral (p = 0.32). There was no correlation between the immunostaining of iNOS and MMP-9 as well as the quantity... (Complete abstract click electronic access below)
We studied the effects of ketorolac tromethamine at 0.5% without preservative, about the time of epithelialization, the clinical, histological (HE) on the expression of inducible Nitric Oxide Synthase (iNOS) and metalloproteinase-9 (MMP -9) in ulcerative keratitis in rabbits caused by alkali. Twelve eyes were treated (GT) to the drug and 12 with saline (GC), immediately to the occurrence of ulcers and every 6 hours. After 24h, six corneas (n = 6) of each group were harvested (first moment). The other (n = 6), were after reepithelialization (second time). In both instances, we evaluated the inflammatory infiltrate and conditions of the newly formed epithelium (HE). For immunohistochemistry, immunostaining of iNOS and MMP-9 was assessed. The average time of epithelialization in GT was 55 hours. In the GC, it was 44 hours (p <0.05). At 24 h, the corneas of GT showed lower inflammatory exudation (p <0.01) compared to the CG. In the second phase, the GT revealed mild inflammatory exudation (p> 0.05) and simple epithelial layer. The GC showed greater stratification in the newly formed epithelium. The average immunostaining of iNOS in stromal cells was higher in GT, the first and second moments compared to control group (p> 0.05). In the second step, the central region expressed more iNOS compared to peripheral in both TG (p <0.01) and in control group (p <0.05). In the epithelium, there were no significant differences in scores of iNOS immunostaining between groups and time (p = 0.69). The scores of immunostaining for MMP-9 in the epithelium were lower in GT compared to GC (p = 0.69). The average immunostaining of MMP-9 in stromal cells, the second time was greater in the central region as compared to the peripheral (p = 0.32). There was no correlation between the immunostaining of iNOS and MMP-9 as well as the quantity... (Complete abstract click electronic access below)
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Palavras-chave
Coelho, Olhos - Ulceras, Córnea, Metaloproteinase 9 da Matriz, Fármacos, Oftalmologia veterinaria, Veterinary ophthalmology
Como citar
LIMA, Tiago Barbalho. Imunomarcação da óxido nítrico sintase induzível e da metaloproteinase-9 em úlceras corneais químicas de coelhos (Oryctolagus cuniculus - Linnaeus, 1758), tratadas com cetorolaco de trometamina. 2013. xii, 50 p. Dissertação (mestrado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal, 2013.