Aplicação de extratos enzimáticos fúngicos na ensilagem do milho
Carregando...
Data
2012-02-24
Autores
Lellis, André Teixeira [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Estadual Paulista (Unesp)
Resumo
Objetivou-se produzir enzimas fibrolíticas por fermentação em estado sólido com os fungos Trichoderma reesei e Thermoascus aurantiacus e avaliar a ensilagem do milho com adição de doses dos extratos enzimáticos produzidos (0; 143,4; 286,8 e 573,6 U CMCase/Kg de matéria natural e 0; 1800,9; 3601,8 e 7203,6 U CMCase//Kg de matéria natural, respectivamente), em delineamento interamente casualizado, com quatro repetições para cada tratamento. Avaliou-se o valor nutritivo da forragem antes da ensilagem e após a abertura dos silos. Após 168h de fermentação, em substrato contendo bagaço de cana-de-açúcar e farelo de trigo (1:1), foi retirado cada extrato enzimático bruto. O extrato do fungo T. reesei continha 14,34 U/mL de CMCase, 1,03 U/mL de β– glicosidase, 74,60 U/mL de Xilanase e 0,48 U/mL de β–xilosidase e do T. aurantiacus continha 180,91 U/mL de CMCase, 6,02 U/mL de β–glicosidase, 719,98 U/mL de Xilanase e 0,17 U/mL de β–xilosidase. A adição dos extrato enzimáticos dos fungos T. reesei e T. aurantiacus, possibilitou queda na recuperação de matéria seca, com Y=96,02–0,1205x (R²=0,48) e Y=95,625– 0,1262x (R²=0,56), aumento nas perdas por gás com Y=23,27+0,0843x (R²=0,29) e Y=19,983+0,2863x (R²=0,39) e queda no teor de N-NH3/NT com Y=3,9243– 0,0434x (R2=0,54) e Y=3,355–0,141x+0,0028x2 (R2=0,56) respectivamente. Também se observou menor recuperação de FDN com Y=72,611–0,1247x (R²=0,52) e Y=74,819–0,1683x (R²=0,50) e FDA com Y=89,841–0,2642x (R²=0,53) e Y=97,181–0,3717x (R²=0,72) para os fungos T. reesei e T. aurantiacus, respectivamente. Como conseqüência propiciou menores valores de DIVMS com Y=75,364–0,1034x (R²=0,51) para o T. aurantiacus. Esses valores podem ser atribuídos pela atuação das enzimas presentes no extrato, removendo barreiras estruturais, com um maior aproveitamento do conteúdo celular pelas bactérias fermentadoras no material ensilado
The aim of this research is to product fibrolitic enzymes by solid state fermentation with Trichoderma reesei and Thermoascus aurantiacus fungi and to analyze the corn ensiling with crescent concentrations of enzymatic extracts produced (0; 143,4; 286,8 e 573,6 U CMCase/Kg of wet material and 0; 1800,9; 3601,8 e 7203,6 U CMCase//Kg of wet material, respectively), in delineation completely casualized, with four replications for each treatment. We evaluated the nutritional value of forage was measured before and after opening silos. After 168h of fermentation using sugar cane bagasse and wheat bran (1:1) as substrate it was collected each crude enzymatic extract. The T. reesei crude extract contained 14,34U/ml of CMCase, 1,03 U/mL of β–glycosidase, 74,60 U/mL of Xylanase and 0,48 U/mL de β–xylosidase. The T. aurantiacus crude extract contained 180,91 U/mL of CMCase, 6,02 U/mL of β–glycosidase, 719,98 U/mL de Xylanase and 0,17 U/mL de β–xylosidase. The addiction of fungus enzymatic extract of T. reesei and T. aurantiacus enabled the overthrow in the recuperation of dry matter, with Y=96,02–0,1205x (R²=0,48) and Y=95,625– 0,1262x (R²=0,56), increase in waste by gas with Y=23,27+0,0843x (R²=0,29) and Y=19,983+0,2863x (R²=0,39) and downfall in the N-NH3/NT content with Y=3,9243–0,0434x (R2=0,54) and Y=3,355–0,141x+0,0028x2 (R2=0,56) respectively. Furthermore, it was observed less recuperation of NDF with Y=72,611–0,1247x (R²=0,52) and Y=74,819–0,1683x (R²=0,50) and ADF with Y=89,841–0,2642x (R²=0,53) and Y=97,181–0,3717x (R²=0,72) for T. reesei and T. aurantiacus, respectively. As a consequence, it propitiated lower values of DIVMS with Y=75,364–0,1034x (R²=0,51) for T. aurantiacus. These values may be attributed by the action of the enzymes present in crude extract, removing structural barriers, with a major use of the cellular content by bacteria in the silage material
The aim of this research is to product fibrolitic enzymes by solid state fermentation with Trichoderma reesei and Thermoascus aurantiacus fungi and to analyze the corn ensiling with crescent concentrations of enzymatic extracts produced (0; 143,4; 286,8 e 573,6 U CMCase/Kg of wet material and 0; 1800,9; 3601,8 e 7203,6 U CMCase//Kg of wet material, respectively), in delineation completely casualized, with four replications for each treatment. We evaluated the nutritional value of forage was measured before and after opening silos. After 168h of fermentation using sugar cane bagasse and wheat bran (1:1) as substrate it was collected each crude enzymatic extract. The T. reesei crude extract contained 14,34U/ml of CMCase, 1,03 U/mL of β–glycosidase, 74,60 U/mL of Xylanase and 0,48 U/mL de β–xylosidase. The T. aurantiacus crude extract contained 180,91 U/mL of CMCase, 6,02 U/mL of β–glycosidase, 719,98 U/mL de Xylanase and 0,17 U/mL de β–xylosidase. The addiction of fungus enzymatic extract of T. reesei and T. aurantiacus enabled the overthrow in the recuperation of dry matter, with Y=96,02–0,1205x (R²=0,48) and Y=95,625– 0,1262x (R²=0,56), increase in waste by gas with Y=23,27+0,0843x (R²=0,29) and Y=19,983+0,2863x (R²=0,39) and downfall in the N-NH3/NT content with Y=3,9243–0,0434x (R2=0,54) and Y=3,355–0,141x+0,0028x2 (R2=0,56) respectively. Furthermore, it was observed less recuperation of NDF with Y=72,611–0,1247x (R²=0,52) and Y=74,819–0,1683x (R²=0,50) and ADF with Y=89,841–0,2642x (R²=0,53) and Y=97,181–0,3717x (R²=0,72) for T. reesei and T. aurantiacus, respectively. As a consequence, it propitiated lower values of DIVMS with Y=75,364–0,1034x (R²=0,51) for T. aurantiacus. These values may be attributed by the action of the enzymes present in crude extract, removing structural barriers, with a major use of the cellular content by bacteria in the silage material
Descrição
Palavras-chave
Enzimas, Forragem, Fungos, Milho - Ensilagem, Corn ensiling - Enzymatic extracts
Como citar
LELLIS, André Teixeira. Aplicação de extratos enzimáticos fúngicos na ensilagem do milho. 2012. xv, 62 f. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, 2012.