Estudos histopatológicos e ciclos biológicos de Meloidogyne mayaguensis e M. javanica em tomateiros com gene Mi
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Data
2010-01-29
Autores
Westerich, Juliana Nogueira [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
A espécie Meloidogyne mayaguensis vem causando grande preocupação por ser capaz de vencer a resistência de tomateiros portadores do gene Mi. O objetivo do trabalho foi estudar o ciclo biológico e a histopatologia de M. mayaguensis em tomateiros resistentes a meloidoginose e compará-los com os aspectos biológicos e histopatológicos de Meloidogyne javanica nas mesmas plantas. O delineamento experimental utilizado foi inteiramente casualizado, sendo para o estudo do ciclo biológico composto de vinte tratamentos (dois porta-enxertos de tomateiro, ‘Magnet’ e ‘Helper M’; duas espécies de nematoides das galhas, M. mayaguensis e M. javanica; e cinco épocas de coleta, 3 DAI, 10 DAI, 17 DAI, 24 DAI e 31 DAI), com cinco repetições. Para o estudo da histopatologia, composto por 12 tratamentos (dois porta-enxertos de tomateiro, ‘Magnet’ e ‘Helper M’; duas espécies de nematoides das galhas, M. mayaguensis e M. javanica; e três épocas de coleta, 10 DAI, 17 DAI e 24 DAI), com três repetições. As plantas foram inoculadas com 500 juvenis infectivos (J2) de M. mayaguensis ou M. javanica. As avaliações foram realizadas em cinco épocas de coleta. Em cada coleta, cinco plantas foram retiradas ao acaso e suas raízes separadas em duas porções. Uma das partes foi submetida à coloração com Fucsina Ácida e dissecada sob microscópio estereoscópico para a contagem dos diferentes estádios de desenvolvimento dos nematoides. Os dados foram submetidos à análise de variância e as médias comparadas pelo teste de Tukey a 5% de probabilidade. Na outra porção do sistema radicular, foram feitos cortes histológicos transversais com 8 μm de espessura, corados com Floxina B seguido de Azul de Toluidina 0,05%, para determinação dos números e áreas das células gigantes e dos sítios de alimentação presentes no cilindro vascular da raiz. Os dados obtidos foram submetidos...
The species Meloidogyne mayaguensis is causing great concern for being able to overcome the resistance of tomatoes carrying the Mi gene. The objective of this work was to study the life cycle and the histopathological changes of M. mayaguensis in nematode-resistant tomatoes and compare them with biological and histopathological aspects of Meloidogyne javanica in the same plants. The experimental design was completely randomized, and to study the life cycle composed of twenty treatments (two varieties of tomato, 'Magnet' and 'Helper M', two species of nematodes, M. javanica and M. mayaguensis and five harvest dates, 3 DAI, 10 DAI 17 DAI 24 DAI and 31 DAI), with five replicates. To study the histopathology, composed of 12 treatments (two varieties of tomato, 'Magnet' and 'Helper M', two species of nematodes, M. javanica and M. mayaguensis and three harvest dates, 10 DAI, 17 DAI and 24 DAI), with three replications. The plants were inoculated with 500 infective juveniles (J2) of M. mayaguensis or M. javanica. The evaluations were conducted in five sampling times. At each harvest, five plants were taken at random and their roots separated into two parts. The first was stained with Acid Fuchsin and dissected under a stereomicroscope to count the different developmental stages of nematodes. The data were subjected to analysis of variance and means compared by Tukey test at 5% probability. In the another portion of the root system, 8 microns thick transversal sections were obtained, stained with Phloxine B followed by Toluidine Blue 0.05%, for determining the numbers and areas of giant cells and feeding sites in the vascular cylinder. The data were subjected to Multivariate Cluster Analysis and Principal Component Analysis. The results showed that the species M. mayaguensis was able to develop normally in the root-stocks, completing its cycle 24 days after inoculation (DAI)... (Complete abstract click electronic access below)
The species Meloidogyne mayaguensis is causing great concern for being able to overcome the resistance of tomatoes carrying the Mi gene. The objective of this work was to study the life cycle and the histopathological changes of M. mayaguensis in nematode-resistant tomatoes and compare them with biological and histopathological aspects of Meloidogyne javanica in the same plants. The experimental design was completely randomized, and to study the life cycle composed of twenty treatments (two varieties of tomato, 'Magnet' and 'Helper M', two species of nematodes, M. javanica and M. mayaguensis and five harvest dates, 3 DAI, 10 DAI 17 DAI 24 DAI and 31 DAI), with five replicates. To study the histopathology, composed of 12 treatments (two varieties of tomato, 'Magnet' and 'Helper M', two species of nematodes, M. javanica and M. mayaguensis and three harvest dates, 10 DAI, 17 DAI and 24 DAI), with three replications. The plants were inoculated with 500 infective juveniles (J2) of M. mayaguensis or M. javanica. The evaluations were conducted in five sampling times. At each harvest, five plants were taken at random and their roots separated into two parts. The first was stained with Acid Fuchsin and dissected under a stereomicroscope to count the different developmental stages of nematodes. The data were subjected to analysis of variance and means compared by Tukey test at 5% probability. In the another portion of the root system, 8 microns thick transversal sections were obtained, stained with Phloxine B followed by Toluidine Blue 0.05%, for determining the numbers and areas of giant cells and feeding sites in the vascular cylinder. The data were subjected to Multivariate Cluster Analysis and Principal Component Analysis. The results showed that the species M. mayaguensis was able to develop normally in the root-stocks, completing its cycle 24 days after inoculation (DAI)... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Tomate, Meloidogyne, Histopatologia, Meloidogyne mayaguensis, Solanum lycopersicum, Biological cycle, Histopathology. eng
Como citar
WESTERICH, Juliana Nogueira. Estudos histopatológicos e ciclos biológicos de Meloidogyne mayaguensis e M. javanica em tomateiros com gene Mi. 2010. viii, 83f. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Ciências Agronômicas de Botucatu, 2010.