Pseudomonas cichorii em tomateiro: ocorrência no Estado de São Paulo, gama de hospedeiras e reação de genótipos
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Data
2007-06-20
Autores
Silva Júnior, Tadeu Antônio Fernandes da [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
Recentemente, em dois campos comerciais de tomateiro dos tipos Salada e Italiano, localizados respectivamente em Bragança Paulista e Mogi Guaçú, SP, foram observados sintomas de queima generalizada nas folhas. Em observações ao microscópio óptico de tecidos infectados foi constatada a presença de exsudação bacteriana. Isolamentos realizados em meio de cultura permitiram obter bactérias com formato bastonete, Gramnegativas, com colônias de coloração branca e produtoras de pigmento fluorescente em meio B de King. Isolados bacterianos foram submetidos a testes bioquímicos e fisiológicos, entre eles, LOPAT, sendo enquadrados no grupo III de LOPAT (- + - - +) e, portanto, identificados como sendo Pseudomonas cichorii. Esses resultados foram corroborados por testes serológicos de imunofluorescência indireta, com antissoros produzidos para isolado tipo de P. cichorii. Esta bactéria causa doença em várias culturas de importância econômica e ainda não havia sido constatada em nosso país, na cultura do tomateiro. Isolados bacterianos encontramse depositados na Coleção de Culturas de Fitobactérias do Instituto Biológico, sob os números de acesso IBSBF 2309 e IBSBF 2323. Foram desenvolvidos também estudos visando a determinação da gama de hospedeiras e a reação de 28 genótipos de tomateiro aos isolados de P. cichorii. Plantas de abobrinha, alface, beldroega, berinjela, beterraba, cenoura, couvebrócolo, datura, fumo, girassol, jiló, melão, pepino, petúnia, pimentão, rabanete, repolho, rúcula, salsa e tomateiro, no estágio de um par de folhas verdadeiras, foram inoculadas por pulverização com os isolados IBSBF 2309 e IBSBF 2323 e um isolado de P. cichorii de girassol (GIR-1). Os isolados IBSBF 2309 e IBSBF 2323 mostraram-se patogênicos à beldroega, à datura, ao girassol, ao pimentão e ao tomateiro, enquanto que o isolado de girassol foi...
Recently, generalized blight symptoms were observed in tomato leaves of the Salada and Italiano types, in two commercial fields located, respectively, in Bragança Paulista and Mogi Guaçú, SP, Brazil. The presence of bacterial exudation was verified in observations of infected tissues under the optical microscope. Rod-shaped, Gram-negative bacteria were obtained from isolations in culture medium; the colonies were white and produced fluorescent pigment in King's B medium. Bacterial isolates were submitted to biochemical and physiological tests, including LOPAT, and were classified into LOPAT group III (- + - - +); consequently, they were identified as Pseudomonas cichorii. These results were corroborated by indirect immunofluorescence tests, using antisera produced for the type isolate of P. cichorii. This bacterium causes diseases in several crops of economic importance and had not yet been observed in tomato in Brasil. Bacterial isolates were deposited in Phytobacteria Culture Collection of Instituto Biológico, under accession numbers IBSBF 2309 and IBSBF 2323. Studies were also carried out in order to determine the host range and reaction of 28 tomato genotypes to P. cichorii isolates. Caserta pumpkin, lettuce, purslane, eggplant, beet, broccoli, carrot, Jimson weed, sunflower, tobacco, scarlet eggplant, melon, cucumber, petunia, green pepper, radish, cabbage, arugula, parsley, and tomato plants, all with one pair of true leaves, were spray-inoculated with isolates IBSBF 2309 and IBSBF 2323 and one P. cichorii isolate from sunflower (GIR-1). Isolates IBSBF 2309 and IBSBF 2323 were pathogenic to purslane, Jimson weed, sunflower, green pepper, and tomatoe, while the sunflower isolate was only pathogenic to purslane, Jimson weed, and sunflower, but not to green pepper or... (Complete abstract click electronic access below)
Recently, generalized blight symptoms were observed in tomato leaves of the Salada and Italiano types, in two commercial fields located, respectively, in Bragança Paulista and Mogi Guaçú, SP, Brazil. The presence of bacterial exudation was verified in observations of infected tissues under the optical microscope. Rod-shaped, Gram-negative bacteria were obtained from isolations in culture medium; the colonies were white and produced fluorescent pigment in King's B medium. Bacterial isolates were submitted to biochemical and physiological tests, including LOPAT, and were classified into LOPAT group III (- + - - +); consequently, they were identified as Pseudomonas cichorii. These results were corroborated by indirect immunofluorescence tests, using antisera produced for the type isolate of P. cichorii. This bacterium causes diseases in several crops of economic importance and had not yet been observed in tomato in Brasil. Bacterial isolates were deposited in Phytobacteria Culture Collection of Instituto Biológico, under accession numbers IBSBF 2309 and IBSBF 2323. Studies were also carried out in order to determine the host range and reaction of 28 tomato genotypes to P. cichorii isolates. Caserta pumpkin, lettuce, purslane, eggplant, beet, broccoli, carrot, Jimson weed, sunflower, tobacco, scarlet eggplant, melon, cucumber, petunia, green pepper, radish, cabbage, arugula, parsley, and tomato plants, all with one pair of true leaves, were spray-inoculated with isolates IBSBF 2309 and IBSBF 2323 and one P. cichorii isolate from sunflower (GIR-1). Isolates IBSBF 2309 and IBSBF 2323 were pathogenic to purslane, Jimson weed, sunflower, green pepper, and tomatoe, while the sunflower isolate was only pathogenic to purslane, Jimson weed, and sunflower, but not to green pepper or... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Pseudomonas cichorii, Tomates - Doenças, Bacterias fitopatogenicas, Tomatoes - Diseases, Phytopathogenic bacteria
Como citar
SILVA JÚNIOR, Tadeu Antônio Fernandes da. Pseudomonas cichorii em tomateiro: ocorrência no Estado de São Paulo, gama de hospedeiras e reação de genótipos. 2007. viii, 60 f. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Ciências Agronômicas, 2007.