Estudos citogenéticos em raias do gênero Patamotrygon (Chondrichthyes: Myliobatiformes: Potamotrygonidae) na bacia superior do rio Paraná
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2009-03-06
Autores
Cruz, Vanessa Paes [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
As raias da família Potamotrygonidae são importantes componentes da ictiofauna Neotropical, sendo este grupo de organismos completamente restrito aos sistemas fluviais da América do Sul. Três gêneros são relacionados nessa família: Plesiotrygon, Paratrygon e Potamotrygon e são encontrados nos principais sistemas fluviais na região Neotropical. No presente projeto, foi realizada a análise citogenética em representantes de duas espécies de raias, Potamotrygon motam e P. falkneri, que apresentaram números diplóide de 2n=65 nos machos e NF= 66 nas temeas, caracterizando a ocorrencia de heteromorfismo cromoss6mico sexual do tipo X1X1X2X2/X1X2Y. Em P. motam a populagao de Porto Rico apresentou numero fundamental de 116 (22m+8sm+20st+16a) nas femeas, e 114 (21m+9sm+19st+16a) nos machos. A populagao de Ilha solteira apresentou numero fundamental 122 (20m+1 Osm+26st+1 Oa) nas fêmeas e 120 (19m+11 sm+25st+1 Oa) nos machos. Em P. falkneri, a população de Porto Rico apresentou numero fundamental 110 (20m+10sm+14st+22a) nas fêmeas e 108 (19m+10sm+14st+22a) nos machos. A população de Ilha Solteira apresentou número fundamental de 114 (20m+10sm+18st+18a) as fêmeas e 112 (19m+10sm+18st+18a) os machos. A heterocromatina constitutiva (Banda C) foi identificada na forma de blocos heterocromáticos nas regiões centroméricas de quase todos os cromossomos do complemento. As regiões organizadoras de nucléolos (RONs) caracterizadas nesta impregnação com nitrato de Prata , se apresentaram em marcações múltiplas e em posição terminal nos cromossomos das duas espécies. 0 emprego do fluorocromo CMA3 evidenciou, além das Ag-RONs, marcações adicionais nas duas espécies em estudo, caracterizando outras regiões no genoma também ricas em GC. Foram identificados também cístrons ribossômicos através da hibridação in situ fluorescente (FISH) com a sonda de DNAr 18S, que coincidiu...
Stingrays of the family Potamotrygonidae are important components of the Neotropical ichthyofauna, being the only group of elasmobranchs completely restricted to fluvial systems of South America. Three genera are recognized within the family: Paratrygon, Plesiotrygon, and Potamotrygon. In this project, the cytogenetic analysis was performed in representatives of two species of rays, Potamotrygon motoro and P. falkneri, who had diploid numbers of 2n = 65 in males and 66 in females, characterizing the occurrence of heteromorphic sex chromosome type X1X1X2X2/X1X2Y. In P. motoro the population of Porto Rico presented fundamental number of 116 (22m+8sm+20st+16a) females and 114 (21m+9sm+19st+16a) in males. The population of Solteira Island presents fundamental number 122 (20m+10sm+26st+10a) in females and 120 (19m+11 sm+25st+1 Oa) in males. In P. falkneri, the population of Porto Rico presents fundamental number 110 (20m+10sm+14st+22a) in females and 108 (19m+10sm+14st+22a) in males. The population of Solteira Island presents fundamental number of 114 (20m+10sm+18st+18a) in females and 112 (19m+10sm+18st+18a) in males. Constitutive heterochromatin (C Band) revealed heterochromatic blocks in the centromeric regions of almost all the chromosomes of the complement. The nucleolar organizer regions (NORs) by impregnation with silver nitrate, presented multiple and terminals NORs in the two species. The use of fluorochrome CMA3 showed, in addition to Ag-NORs, additional marks in the two studied species, characterizing other regions in the genome rich in GC. Were also identified cistrons ribosomic by fluorescent in situ hybridization (FISH) with the probe 18S rDNA, which coincided with the same markings evidenced by the impregnation of silver in the two species, and one additional site was found for the species P. falkneri, population of Solteira Island. The probe of 5S rDNA revealed... (Complete abstract click electronic access below)
Stingrays of the family Potamotrygonidae are important components of the Neotropical ichthyofauna, being the only group of elasmobranchs completely restricted to fluvial systems of South America. Three genera are recognized within the family: Paratrygon, Plesiotrygon, and Potamotrygon. In this project, the cytogenetic analysis was performed in representatives of two species of rays, Potamotrygon motoro and P. falkneri, who had diploid numbers of 2n = 65 in males and 66 in females, characterizing the occurrence of heteromorphic sex chromosome type X1X1X2X2/X1X2Y. In P. motoro the population of Porto Rico presented fundamental number of 116 (22m+8sm+20st+16a) females and 114 (21m+9sm+19st+16a) in males. The population of Solteira Island presents fundamental number 122 (20m+10sm+26st+10a) in females and 120 (19m+11 sm+25st+1 Oa) in males. In P. falkneri, the population of Porto Rico presents fundamental number 110 (20m+10sm+14st+22a) in females and 108 (19m+10sm+14st+22a) in males. The population of Solteira Island presents fundamental number of 114 (20m+10sm+18st+18a) in females and 112 (19m+10sm+18st+18a) in males. Constitutive heterochromatin (C Band) revealed heterochromatic blocks in the centromeric regions of almost all the chromosomes of the complement. The nucleolar organizer regions (NORs) by impregnation with silver nitrate, presented multiple and terminals NORs in the two species. The use of fluorochrome CMA3 showed, in addition to Ag-NORs, additional marks in the two studied species, characterizing other regions in the genome rich in GC. Were also identified cistrons ribosomic by fluorescent in situ hybridization (FISH) with the probe 18S rDNA, which coincided with the same markings evidenced by the impregnation of silver in the two species, and one additional site was found for the species P. falkneri, population of Solteira Island. The probe of 5S rDNA revealed... (Complete abstract click electronic access below)
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Raia (Peixe) - Paraná, Rio, Bacia, Peixe - Genetica, Zoologia, Rays, Zoology
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CRUZ, Vanessa Paes. Estudos citogenéticos em raias do gênero Patamotrygon (Chondrichthyes: Myliobatiformes: Potamotrygonidae) na bacia superior do rio Paraná. 2009. 132 f. Dissertação (mestrado) - Universidade Estadual Paulista, Instituto de Biociências de Botucatu, 2009.