Biomphalaria tenagophila: genetic variability within intermediate snail hosts susceptible and resistant to Schistosoma mansoni infection

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dc.contributor.authorDa Silva, D.
dc.contributor.authorSpada, RGM
dc.contributor.authorSobral-Hamaguchi, S. S.
dc.contributor.authorAbdel-Hamid, Z.
dc.contributor.authorZuim, NRB
dc.contributor.authorZanotti-Magalhaes, E. M.
dc.contributor.authorMagalhaes, L. A.
dc.contributor.authorRibeiro-Paes, J. T.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionNatl Res Ctr
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.date.accessioned2014-05-20T13:22:03Z
dc.date.available2014-05-20T13:22:03Z
dc.date.issued2004-03-01
dc.description.abstractDNA analysis by molecular techniques has significantly expanded the perspectives of the study and understanding of genetic variability in molluscs that ere vectors of schistosomiasis. In tire present study, the genetic variability of susceptible and resistant B. tenagophila strains to S. mansoni infection was investigated using amplification of their genomic DNA by RAPD-PCR. The products were analyzed by PAGE and stained with silver. The results showed pdymorphism between tested strains with four different primers. We found two bonds of 1,900 and 3,420 bp that were characteristic of the susceptible strains with primer 2. The primers 9 end 10 identified a single polymorphic bond that was also characteristic of (3,136 and 5,041 bp, respectively) susceptible snails. Two polymorphic bonds were detected by primer 15: one with 1 800 bp was characteristic of the resistant strain and the other with 1,700 do in the susceptible one. These results provide additional evidence showing that the RAPD-PCR technique is adequate for the study of polymorphisms in intermediate hosts snails of S. mansoni. The obtained results are expected to expend the knowledge about the genetic variability of the snails and to permit the future identification of genomic sequences specifically related to the resistance/susceptibility of Biompholario to the larval forms of S. mansoni.en
dc.description.affiliationUNESP, Dept Biol Sci, BR-19806900 Assis, SP, Brazil
dc.description.affiliationNatl Res Ctr, Dept Med Chem, Cairo, Egypt
dc.description.affiliationUniv Estadual Campinas, Inst Biol, Dept Parasitol, Campinas, SP, Brazil
dc.description.affiliationUnespUNESP, Dept Biol Sci, BR-19806900 Assis, SP, Brazil
dc.format.extent43-49
dc.identifier.citationParasite-Journal de La Societe Francaise de Parasitologie. Issy Moulineaux: Princeps Editions, v. 11, n. 1, p. 43-49, 2004.
dc.identifier.issn1252-607X
dc.identifier.urihttp://hdl.handle.net/11449/6435
dc.identifier.wosWOS:000220703100005
dc.language.isoeng
dc.publisherPrinceps Editions
dc.relation.ispartofParasite-Journal de La Societe Francaise de Parasitologie
dc.relation.ispartofjcr2.069
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectbiomphalariapt
dc.subjectschistosomiasispt
dc.subjectRAPD-PCRpt
dc.subjectgenetic variabilitypt
dc.subjectpolymorphismpt
dc.titleBiomphalaria tenagophila: genetic variability within intermediate snail hosts susceptible and resistant to Schistosoma mansoni infectionen
dc.typeArtigo
dcterms.licensehttp://www.edp-open.org/index.php?option=com_content&view=article&id=238&Itemid=100&lang=en_GB.utf8%2C+en_GB.UT
dcterms.rightsHolderPrinceps Editions
unesp.author.orcid0000-0002-7645-5891[8]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências e Letras, Assispt

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Artigos - Ciências Biológicas - FCLAS