Metarhizium anisopliae conidia production in packed-bed bioreactor using rice as substrate in successive cultivations

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dc.contributor.authorda Cunha, Lucas Portilho [UNESP]
dc.contributor.authorCasciatori, Fernanda Perpétua
dc.contributor.authorVicente, Isabela Valente [UNESP]
dc.contributor.authorGarcia, Renata Laurito [UNESP]
dc.contributor.authorThoméo, João Cláudio [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.date.accessioned2020-12-12T02:45:40Z
dc.date.available2020-12-12T02:45:40Z
dc.date.issued2020-10-01
dc.description.abstractThis paper presents a study on scale-up and cost reduction of the production of spores of Metarhizium anisopliae IBCB 425, entomopathogenic fungus used in sugarcane crops. Rice was mixed with sugarcane bagasse (9:1 w/w) for substrate composition, assuring adequate physical structure for cultivation in packed-beds. Spores yield from only rice in bench-scale packed-bed bioreactor was 56 % of the one obtained from the mixture 9:1 w/w. In comparison to plastic packages used in bioindustries, equivalent spores yields per gram of substrate have been achieved in bench and pilot-scale bioreactors built by cylindrical jacketed modules, that provide better control of operational and environmental variables, attested by little variability among replicates. Although non-negligible temperature rise (5 °C above the ideal) occurred within the pilot-scale bioreactor, spores production was not harmed in comparison to bench-scale. By reusing rice up to three successive cultivations, a 2.5-fold increase of spores yields was achieved in comparison to single use. Temperatures and CO2 profiles corroborates the fungus adapted differently to substrate at each usage. Such results are valuable for industrial producers of commercial formulations of the fungus spores, allowing process modernization by using packed-bed bioreactors and production costs and rice demand reduction by recycling the substrate.en
dc.description.affiliationGraduate Program in Food Engineering and Science Food Engineering and Technology Department Institute of Biosciences Letters and Exact Sciences São Paulo State University (UNESP), Cristóvão Colombo 2265, Jardim Nazareth
dc.description.affiliationGraduate Program in Chemical Engineering Chemical Engineering Department Center of Exact Sciences and of Technology Federal University of São Carlos (UFSCar), Rod. Washington Luiz km 235 SP 310, Bairro Monjolinho
dc.description.affiliationUnespGraduate Program in Food Engineering and Science Food Engineering and Technology Department Institute of Biosciences Letters and Exact Sciences São Paulo State University (UNESP), Cristóvão Colombo 2265, Jardim Nazareth
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 2010/12624-0
dc.description.sponsorshipIdFAPESP: 2014/23453-3
dc.description.sponsorshipIdFAPESP: 2018/00996-2
dc.description.sponsorshipIdCNPq: 430786/2018-2
dc.format.extent104-111
dc.identifierhttp://dx.doi.org/10.1016/j.procbio.2020.07.002
dc.identifier.citationProcess Biochemistry, v. 97, p. 104-111.
dc.identifier.doi10.1016/j.procbio.2020.07.002
dc.identifier.issn1359-5113
dc.identifier.scopus2-s2.0-85087589803
dc.identifier.urihttp://hdl.handle.net/11449/201935
dc.language.isoeng
dc.relation.ispartofProcess Biochemistry
dc.sourceScopus
dc.subjectBiocontrol
dc.subjectScale-up
dc.subjectSolid-state fermentation
dc.subjectSugarcane crops
dc.titleMetarhizium anisopliae conidia production in packed-bed bioreactor using rice as substrate in successive cultivationsen
dc.typeArtigo

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Artigos - Engenharia e Tecnologia de Alimentos - IBILCE