Localization of 5-methylcytosine in metaphase chromosomes of diploid and triploid pacu fish, Piaractus mesopotamicus (Pisces, Characiformes)

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dc.contributor.authorDe Almeida-Toledo, L. F.
dc.contributor.authorViegas-Péquignot, E.
dc.contributor.authorCoutinho-Barbosa, A. C.
dc.contributor.authorForesti, F. [UNESP]
dc.contributor.authorNiveleau, A.
dc.contributor.authorDe Almeida Toledo-Filho, S.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionHôpital Necker-Enfants Malades
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionCNRS
dc.date.accessioned2022-04-28T19:54:49Z
dc.date.available2022-04-28T19:54:49Z
dc.date.issued1998-12-01
dc.description.abstractThe distribution of 5-methylcytosine (5-MeC) was investigated in fish chromosomes by indirect immunofluorescence using a highly specific 5-MeC monoclonal antibody. Diploid and artificially produced triploid specimens of the pacu fish, Piaractus mesopotamicus, were analyzed. The strong immunofluorescent signals were coincident with the heterochromatic regions of both diploids and triploids in a pattern that matched the C-banding pattern. In the euchromatin, heterogeneous labeling was observed along the chromatids. The weakness of this labeling hindered comparison of the fluorescence labeling of homologous chromosomes from diploid and triploid individuals. However, no striking differences were observed. The possibility that the euchromatin labeling by the 5-MeC antibody is related to the occurrence of mildly repetitive sequences in the genome of Piaractus is discussed.en
dc.description.affiliationDepartamento de Biologia Instituto de Biociências Universidade de São Paulo, São Paulo
dc.description.affiliationU 383 INSERM Hôpital Necker-Enfants Malades, Paris
dc.description.affiliationDepartamento de Morfologia Instituto de Biociências UNESP, Botucatu
dc.description.affiliationInstitut Pasteur CNRS, Lyon
dc.description.affiliationDepartamento de Biologia Instituto de Biociências USP, Calxa Postal 11.461, 05422-970 São Paulo, SP
dc.description.affiliationUnespDepartamento de Morfologia Instituto de Biociências UNESP, Botucatu
dc.format.extent21-24
dc.identifier.citationCytogenetics and Cell Genetics, v. 83, n. 1-2, p. 21-24, 1998.
dc.identifier.issn0301-0171
dc.identifier.scopus2-s2.0-0032434336
dc.identifier.urihttp://hdl.handle.net/11449/224110
dc.language.isoeng
dc.relation.ispartofCytogenetics and Cell Genetics
dc.sourceScopus
dc.titleLocalization of 5-methylcytosine in metaphase chromosomes of diploid and triploid pacu fish, Piaractus mesopotamicus (Pisces, Characiformes)en
dc.typeArtigo

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