Combined effects of temperature and copper on oxygen consumption and antioxidant responses in the mudflat fiddler crab Minuca rapax (Brachyura, Ocypodidae)

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dc.contributor.authorCapparelli, Mariana Vellosa
dc.contributor.authorBordon, Isabella C. [UNESP]
dc.contributor.authorAraujo, Giuliana [UNESP]
dc.contributor.authorGusso-Choueri, Paloma Kachel [UNESP]
dc.contributor.authorSouza Abessa, Denis Moledo de [UNESP]
dc.contributor.authorMcNamara, John Campbell
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Reg Amazon IKIAM
dc.date.accessioned2019-10-04T12:40:00Z
dc.date.available2019-10-04T12:40:00Z
dc.date.issued2019-09-01
dc.description.abstractThis study investigates the combined effects of waterborne copper exposure and acute temperature change on oxygen consumption and the oxidative stress biomarkers, glutathione S-transferase (GST) and glutathione peroxidase (GPx), in the gills and hepatopancreas of the fiddler crab Minuca rapax. Crabs held at 25 degrees C were acclimated to 0 (control), 50, 250 or 500 mu g Cu L-1 for 21 days, and were then subjected to 15, 25 and 35 degrees C for 24 h. Aerial oxygen consumption rates of crabs in copper free media increased with increasing temperature from 15 to 35 degrees C, Q(10) values reaching approximate to 3. Crabs exposed to increasing copper concentrations exhibited variable responses, Q so values falling to approximate to 1.5. Copper had no effect on oxygen consumption at 25 degrees C. However, at 35 degrees C, rates decreased in a clear concentration-response manner in the copper exposed crabs, revealing impaired aerobic capability. At 15 degrees C, oxygen consumption rates increased with copper concentration, except, for a decrease at 500 mu g CuL-1. Gill GST activity was approximate to 2-fold that of the hepatopancreas, while hepatopancreas GPx activity was 3-fold that of the gills. Gill GST activities were reduced by copper exposure only at 25 degrees C while hepatopancreas GST activities were altered by copper at all temperatures. Hepatopancreas GST and GPx activities increased in crabs exposed to copper at 35 degrees C, revealing oxidative stress induction. Hepatopancreas GST and GPx activities were reduced in copper exposed crabs at 15 degrees C, suggesting a diminished capability to mitigate the effects of copper exposure at low temperature. These findings reveal that copper exposure increases oxygen consumption at low temperatures but decreases consumption at high temperature. Hepatopancreas GPx activities decreased at low temperature and increased at high temperature. These novel findings demonstrate that the interaction between copper exposure and temperature should be considered when evaluating biomarker activities in semi-terrestrial crabs.en
dc.description.affiliationUniv Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, BR-14040901 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Campus Sao Vicente, BR-11380972 Sao Vicente, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Ctr Biol Marinha, BR-11600000 Sao Sebastiao, SP, Brazil
dc.description.affiliationUniv Reg Amazon IKIAM, Via Tena,Muyuna Kilometro 7, Tena, Napo, Ecuador
dc.description.affiliationUnespUniv Estadual Paulista, Campus Sao Vicente, BR-11380972 Sao Vicente, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdFAPESP: 2011/22537-0
dc.description.sponsorshipIdFAPESP: 2011/08065-9
dc.description.sponsorshipIdCNPq: 300662/2009-2
dc.description.sponsorshipIdCNPq: 308649/2011
dc.description.sponsorshipIdCAPES: 33002029031P8
dc.description.sponsorshipIdCAPES: 001
dc.format.extent35-41
dc.identifierhttp://dx.doi.org/10.1016/j.cbpc.2019.05.005
dc.identifier.citationComparative Biochemistry And Physiology C-toxicology & Pharmacology. New York: Elsevier Science Inc, v. 223, p. 35-41, 2019.
dc.identifier.doi10.1016/j.cbpc.2019.05.005
dc.identifier.issn1532-0456
dc.identifier.urihttp://hdl.handle.net/11449/185951
dc.identifier.wosWOS:000477686100005
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofComparative Biochemistry And Physiology C-toxicology & Pharmacology
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectOxygen consumption
dc.subjectAnti-oxidant activities
dc.subjectBiomarkers
dc.subjectCopper exposure
dc.subjectTemperature effects
dc.subjectGelasiminae
dc.subjectSemi-terrestrial fiddler crab
dc.titleCombined effects of temperature and copper on oxygen consumption and antioxidant responses in the mudflat fiddler crab Minuca rapax (Brachyura, Ocypodidae)en
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.

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